Gray P W, Barrett K, Chantry D, Turner M, Feldmann M
Charing Cross Sunley Research Centre, Hammersmith, London, England.
Proc Natl Acad Sci U S A. 1990 Oct;87(19):7380-4. doi: 10.1073/pnas.87.19.7380.
The cDNA for one of the receptors for human tumor necrosis factor (TNF) has been isolated. This cDNA encodes a protein of 455 amino acids that is divided into an extracellular domain of 171 residues and a cytoplasmic domain of 221 residues. The extracellular domain has been engineered for expression in mammalian cells, and this recombinant derivative binds TNF alpha with high affinity and inhibits its cytotoxic activity in vitro. The TNF receptor exhibits similarity with a family of cell surface proteins that includes the nerve growth factor receptor, the human B-cell surface antigen CD40, and the rat T-cell surface antigen OX40. The TNF receptor contains four cysteine-rich subdomains in the extracellular portion. Mammalian cells transfected with the entire TNF receptor cDNA bind radiolabeled TNF alpha with an affinity of 2.5 x 10(-9) M. This binding can be competitively inhibited with unlabeled TNF alpha or lymphotoxin (TNF beta).
人类肿瘤坏死因子（TNF）的一种受体的互补DNA（cDNA）已被分离出来。该cDNA编码一个由455个氨基酸组成的蛋白质，它被分为一个由171个残基组成的细胞外结构域和一个由221个残基组成的细胞质结构域。细胞外结构域已被设计用于在哺乳动物细胞中表达，这种重组衍生物以高亲和力结合肿瘤坏死因子α（TNFα）并在体外抑制其细胞毒性活性。TNF受体与一类细胞表面蛋白具有相似性，这类蛋白包括神经生长因子受体、人类B细胞表面抗原CD40和大鼠T细胞表面抗原OX40。TNF受体在细胞外部分含有四个富含半胱氨酸的亚结构域。用完整的TNF受体cDNA转染的哺乳动物细胞以2.5×10⁻⁹ M的亲和力结合放射性标记的TNFα。这种结合可以被未标记的TNFα或淋巴毒素（TNFβ）竞争性抑制。
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