Kohno T, Brewer M T, Baker S L, Schwartz P E, King M W, Hale K K, Squires C H, Thompson R C, Vannice J L
Synergen, Inc., Boulder, CO 80301.
Proc Natl Acad Sci U S A. 1990 Nov;87(21):8331-5. doi: 10.1073/pnas.87.21.8331.
An inhibitor of tumor necrosis factor (TNF) has been isolated from the human histiocytic lymphoma cell line U-937 that is capable of inhibiting both TNF-alpha and TNF-beta. Protein sequencing has verified that it is distinct from a previously described TNF inhibitor that is a soluble fragment of a TNF receptor molecule (TNFrI). The cDNA sequence of this second TNF inhibitor clone suggests that it is also a soluble fragment of a TNF receptor. Expression of this cDNA sequence in COS-7 cells verified that it encodes a receptor for TNF-alpha (TNFrII) that can give rise to a soluble inhibitor of TNF-alpha, presumably through proteolytic cleavage. The extracellular domain of TNFrII has significant homology with that of TNFrI and these two receptors share a striking conservation of cysteine residue alignment with the extracellular domain of the nerve growth factor receptor. These three receptor molecules are therefore members of a family of polypeptide hormone receptors.
已从人组织细胞淋巴瘤细胞系U - 937中分离出一种肿瘤坏死因子（TNF）抑制剂，它能够抑制肿瘤坏死因子α（TNF - α）和肿瘤坏死因子β（TNF - β）。蛋白质测序证实，它与先前描述的一种TNF抑制剂不同，后者是TNF受体分子（TNFrI）的可溶性片段。第二个TNF抑制剂克隆的cDNA序列表明，它也是TNF受体的可溶性片段。该cDNA序列在COS - 7细胞中的表达证实，它编码一种TNF - α受体（TNFrII），该受体可能通过蛋白水解切割产生一种可溶性的TNF - α抑制剂。TNFrII的细胞外结构域与TNFrI的细胞外结构域具有显著的同源性，并且这两种受体与神经生长因子受体的细胞外结构域在半胱氨酸残基排列上具有惊人的保守性。因此，这三种受体分子是多肽激素受体家族的成员。
