Laufs J, Wirtz U, Kammann M, Matzeit V, Schaefer S, Schell J, Czernilofsky A P, Baker B, Gronenborn B
Max-Planck-Institut für Züchtungsforschung, Köln, Federal Republic of Germany.
Proc Natl Acad Sci U S A. 1990 Oct;87(19):7752-6. doi: 10.1073/pnas.87.19.7752.
The maize transposon Activator (Ac) and in vitro-generated nonautonomous derivatives thereof [Ac delta or Dissociation (Ds) elements] were inserted into the genome of a geminivirus of graminaceous plants, wheat dwarf virus, at a site that does not interfere with viral replication. These recombinant viral genomes were introduced into wheat, maize, and rice protoplasts, where rapid and efficient excision of Ac was observed. Excision was detected only in vectors in which, after transfection, the virus could replicate. This result is not restricted to the autonomous Ac; excision of Ds elements was also induced by transposase activity provided in trans by plasmids expressing the cDNA of Ac. The potential of this combination of a transposon with a viral replicon for plant molecular genetic engineering is discussed.
将玉米转座子激活子（Ac）及其体外产生的非自主衍生物[Acδ或解离（Ds）元件]插入到禾本科植物双生病毒小麦矮缩病毒的基因组中，插入位点不影响病毒复制。这些重组病毒基因组被导入小麦、玉米和水稻原生质体中，在其中观察到Ac的快速高效切除。仅在转染后病毒能够复制的载体中检测到切除现象。这一结果并不局限于自主型Ac；表达Ac cDNA的质粒通过反式提供的转座酶活性也能诱导Ds元件的切除。本文讨论了转座子与病毒复制子这种组合在植物分子遗传工程中的潜力。
