离子液体环境中的蛋白质稳定性：差示扫描荧光法的应用。
Protein stability in an ionic liquid milieu: on the use of differential scanning fluorimetry.
机构信息
Instituto Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. República 127, 2780-756 Oeiras, Portugal.
出版信息
Phys Chem Chem Phys. 2011 Aug 14;13(30):13614-6. doi: 10.1039/c1cp21187k. Epub 2011 Jun 27.
PMID:21709866
Abstract
Protein stability is a major bottleneck in the biotechnological application of ionic liquid-containing solvents, either in the frame of biocatalysis or protein storage. Herein, differential scanning fluorimetry was successfully implemented as a high throughput method to fast scan the impact of a number of cholinium-based ionic liquids on the stability of proteins.
摘要
蛋白质稳定性是含离子液体溶剂在生物催化或蛋白质储存框架中的生物技术应用的主要瓶颈。在此，差示扫描荧光法被成功地实施为一种高通量方法，以快速扫描许多胆碱基离子液体对蛋白质稳定性的影响。
Zotero 插件