Department of Chemistry and Biochemistry, Rowan University, Glassboro, NJ 08028, USA.
Department of Biological Sciences, Rowan University, Glassboro, NJ 08028, USA.
Molecules. 2021 Jan 19;26(2):514. doi: 10.3390/molecules26020514.
In the past decade, innovative protein therapies and bio-similar industries have grown rapidly. Additionally, ionic liquids (ILs) have been an area of great interest and rapid development in industrial processes over a similar timeline. Therefore, there is a pressing need to understand the structure and function of proteins in novel environments with ILs. Understanding the short-term and long-term stability of protein molecules in IL formulations will be key to using ILs for protein technologies. Similarly, ILs have been investigated as part of therapeutic delivery systems and implicated in numerous studies in which ILs impact the activity and/or stability of protein molecules. Notably, many of the proteins used in industrial applications are involved in redox chemistry, and thus often contain metal ions or metal-associated cofactors. In this review article, we focus on the current understanding of protein structure-function relationship in the presence of ILs, specifically focusing on the effect of ILs on metal containing proteins.
在过去的十年中，创新型蛋白质疗法和生物类似药行业发展迅速。此外，在类似的时间范围内，离子液体（ILs）在工业过程中也是一个备受关注和快速发展的领域。因此，迫切需要了解蛋白质在新型环境中的结构和功能。了解蛋白质分子在 IL 制剂中的短期和长期稳定性对于将 IL 用于蛋白质技术至关重要。同样，IL 已被作为治疗性递药系统的一部分进行研究，并在许多研究中表明 IL 会影响蛋白质分子的活性和/或稳定性。值得注意的是，许多用于工业应用的蛋白质都涉及氧化还原化学，因此通常含有金属离子或金属相关辅助因子。在这篇综述文章中，我们重点介绍了目前对 IL 存在下蛋白质结构-功能关系的理解，特别是关注 IL 对含金属蛋白质的影响。
