Wiggins R E, Jafri M S, Proia A D
Department of Ophthalmology, Duke University Medical Center, Durham, North Carolina.
Prostaglandins. 1990 Aug;40(2):131-41. doi: 10.1016/0090-6980(90)90079-b.
12(R)-hydroxy-5,8,10,14-eicosatetraenoic acid [12(R)-HETE] is reported to be more potent than its epimer 12(S)-HETE as a chemoattractant for human neutrophils in vitro and following topical application to the skin. To assess the in vivo neutrophil chemoattractant potencies of 12(S)-HETE and 12(R)-HETE in the rat, we injected 1 microgram, 5 micrograms, or 10 micrograms of these eicosanoids into the corneal stroma. Rats were killed 12-15 hours after injection, and the number of neutrophils in the stroma was counted in a histological section of the cornea including the injection site. The number of neutrophils was significantly increased in corneas injected with 5 micrograms (+103% of control) or 10 micrograms (+456% of control) of 12(S)-HETE and in those injected with 10 micrograms of 12(R)-HETE (+111% of control). The neutrophilic infiltrate in corneas injected with 1 microgram or 5 micrograms of 12(S)-HETE was not significantly different from that in corneas injected with 1 microgram of leukotriene B4. The data for the 10 micrograms injections indicate that 12(S)-HETE is a more potent neutrophil chemoattractant than 12(R)-HETE in the rat cornea. Our results suggest that species or tissue specificity may determine the relative potencies of 12-HETE epimers as chemoattractants for neutrophils, and that 12(S)-HETE may be an important inflammatory mediator in the rat cornea.
据报道，12（R）-羟基-5,8,10,14-二十碳四烯酸[12（R）-HETE]作为人中性粒细胞的趋化因子，在体外和局部应用于皮肤后，其活性比其差向异构体12（S）-HETE更强。为了评估12（S）-HETE和12（R）-HETE在大鼠体内对中性粒细胞的趋化活性，我们将1微克、5微克或10微克的这些类二十烷酸注射到角膜基质中。注射后12 - 15小时处死大鼠，并在包括注射部位的角膜组织切片中计数基质中的中性粒细胞数量。注射5微克（比对照组增加103%）或10微克（比对照组增加456%）的12（S）-HETE以及注射10微克12（R）-HETE（比对照组增加111%）的角膜中，中性粒细胞数量显著增加。注射1微克或5微克12（S）-HETE的角膜中的嗜中性粒细胞浸润与注射1微克白三烯B4的角膜中的嗜中性粒细胞浸润无显著差异。10微克注射的数据表明，在大鼠角膜中，12（S）-HETE是比12（R）-HETE更强的中性粒细胞趋化因子。我们的结果表明，物种或组织特异性可能决定12-HETE差向异构体作为中性粒细胞趋化因子的相对活性，并且12（S）-HETE可能是大鼠角膜中的一种重要炎症介质。
