Department of Trauma and Orthopaedic Surgery, University of Rome “Tor Vergata”, School of Medicine, Italy.
Eur Cell Mater. 2011 Jun 20;21:548-57. doi: 10.22203/ecm.v021a41.
We evaluated the expression of several genes involved in tissue remodelling and bone development in patients with calcific tendinopathy of the rotator cuff. Biopsies from calcified and non-calcified areas were obtained from 10 patients (8 women and 2 men; average age: 55 years; range: 40-68) with calcific tendinopathy of the rotator cuff. To evaluate the expression of selected genes, RNA extraction, cDNA synthesis and quantitative polymerase chain reaction (PCR) were performed. A significantly increased expression of tissue transglutaminase (tTG)2 and its substrate, osteopontin, was detected in the calcific areas compared to the levels observed in the normal tissue from the same subject with calcific tendinopathy, whereas a modest increase was observed for catepsin K. There was also a significant decrease in mRNA expression of Bone Morphogenetic Protein (BMP)4 and BMP6 in the calcific area. BMP-2, collagen V and vascular endothelial growth factor (VEGF) did not show significant differences. Collagen X and matrix metalloproteinase (MMP)-9 were not detectable. A variation in expression of these genes could be characteristic of this form tendinopathy, since an increased level of these genes has not been detected in other forms of tendon lesions.
我们评估了肩袖钙化性肌腱炎患者组织重塑和骨发育相关的几个基因的表达。从 10 名肩袖钙化性肌腱炎患者（8 名女性，2 名男性；平均年龄：55 岁；范围：40-68 岁）的钙化和非钙化区域获取活检。为了评估选定基因的表达，进行了 RNA 提取、cDNA 合成和定量聚合酶链反应（PCR）。与来自同一患有钙化性肌腱炎患者的正常组织相比，在钙化区域中检测到组织转谷氨酰胺酶（tTG）2 及其底物骨桥蛋白的表达显著增加，而组织蛋白酶 K 的表达略有增加。在钙化区域中，骨形态发生蛋白（BMP）4 和 BMP6 的 mRNA 表达也显著降低。BMP-2、胶原 V 和血管内皮生长因子（VEGF）没有显示出显著差异。胶原 X 和基质金属蛋白酶（MMP）-9 无法检测到。这些基因的表达变化可能是这种形式肌腱病的特征，因为在其他形式的肌腱病变中未检测到这些基因的水平增加。
