Medri C, Ruas E A, Medri M E, Ruas C F, Sayhun S, Medri P S, Silva D C G, Bianchini E, Ruas P M
Centro de Ciências Biológicas, Universidade Estadual do Norte do Paraná, Campus Luiz Meneghel, Bandeirantes, PR, Brasil.
Genet Mol Res. 2011 Jun 7;10(2):1084-91. doi: 10.4238/vol10-2gmr1044.
Saplings of Aegiphila sellowiana were submitted to flooding and analysis of genetic diversity in order to investigate flooding tolerance as well as its genetic determination. This response is important because it means that some lines could be planted in degraded riparian areas. Leaves were sampled from each plant, and they were submitted to different flooding periods. Mortality of saplings was 40, 80, 50, 53.3, 33.3, and 33.3% in flooding for 15, 18, 25, 50, 80 days, and flooding for 50 days followed by re-aeration for 30 days, respectively. From the total number of flooded plants, 46.7% died in the first seven days of treatment, while 53.3% survived the flooding. The percentage of polymorphic loci (P(p)), Nei's genetic diversity (H) and the Shannon index (I) were slightly higher for the group that survived the stress of flooding (surviving: P(p) (%) = 67.48, H = 0.184, I = 0.287; not surviving: P(p) (%) = 66.67, H = 0.165, I = 0.261). Analysis of molecular variance showed that 5.88% of the genetic variability was due to the differences between groups of plants surviving and not surviving flooding, while 94.12% was due to genetic differences between individuals within these groups. Similar results were obtained by principal coordinate analysis. Based on these results, we can assume the existence of environment-specific genotypes and the genetic determination of flooding tolerance in A. sellowiana. Thus, some lines of A. sellowiana could be used in the reforestation of riparian habitats, especially in uplands along riverbanks.
为了研究黄杨叶拟美花（Aegiphila sellowiana）的耐淹性及其遗传决定因素，对其树苗进行了淹水试验和遗传多样性分析。这种反应很重要，因为这意味着一些品系可以种植在退化的河岸地区。从每株植物上采集叶片，并使其遭受不同的淹水时间。在分别淹水15天、18天、25天、50天、80天以及淹水50天后再通气30天的情况下，树苗的死亡率分别为40%、80%、50%、53.3%、33.3%和33.3%。在淹水的植株总数中，46.7%在处理的前七天死亡，而53.3%在淹水后存活下来。对于在淹水胁迫中存活下来的群体，多态位点百分比（P(p)）、内氏遗传多样性（H）和香农指数（I）略高（存活：P(p)（%）=67.48，H = 0.184，I = 0.287；未存活：P(p)（%）=66.67，H = 0.165，I = 0.261）。分子方差分析表明，5.88%的遗传变异是由于淹水存活和未存活植株群体之间的差异，而94.12%是由于这些群体内个体之间的遗传差异。主坐标分析也得到了类似的结果。基于这些结果，我们可以假设存在特定环境的基因型以及黄杨叶拟美花耐淹性的遗传决定因素。因此，一些黄杨叶拟美花品系可用于河岸栖息地的重新造林，特别是在河岸高地。
