Caffara Monica, Locke Sean A, Gustinelli Andrea, Marcogliese David J, Fioravanti Maria L
Department of Veterinary Medical Sciences, Alma Mater Studiorum Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy.
J Parasitol. 2011 Oct;97(5):884-91. doi: 10.1645/GE-2781.1. Epub 2011 Jun 28.
The separation of Clinostomum complanatum Rudolphi, 1814 and Clinostomum marginatum Rudolphi, 1819 has long been unclear. Recent data confirm the validity of the junior species, C. marginatum , by ∼ 1% differences in its 18S rDNA sequences. We collected adults and metacercariae of C. complanatum and C. marginatum and found reliable morphological differences in the genital complex at both developmental stages. In addition, we identified basic morphometrics (distance between suckers, body width) in metacercariae that may be useful for discriminating the species. The morphological differences were supported by the comparison of sequences of internal transcribed spacers of ribosomal DNA and of the mitochondrial gene cytochrome c oxidase I (COI) from 39 specimens. In 36 specimens, the average divergence between the species was 7.3% in ITS and 19.4% in COI sequences. Two specimens from North America and 1 from Europe had sequences that did not allow them to be clearly allied with either species.
1814年鲁道夫描述的扁体 Clinostomum complanatum 与1819年鲁道夫描述的边体 Clinostomum marginatum 之间的分类长期以来一直不明确。最近的数据通过其18S rDNA序列约1%的差异证实了较新物种边体 Clinostomum marginatum 的有效性。我们收集了扁体 Clinostomum complanatum 和边体 Clinostomum marginatum 的成虫和囊蚴，发现在两个发育阶段的生殖复合体存在可靠的形态差异。此外，我们确定了囊蚴的基本形态测量指标（吸盘间距离、体宽），这些指标可能有助于区分这两个物种。核糖体DNA内转录间隔区和39个样本的线粒体基因细胞色素c氧化酶I（COI）序列的比较支持了形态学差异。在36个样本中，两个物种之间的ITS平均差异为7.3%，COI序列平均差异为19.4%。来自北美的两个样本和来自欧洲的一个样本的序列无法使其与任何一个物种明确关联。
