应用实时荧光定量PCR和酶联免疫吸附测定法检测马流感病毒，以确定自然感染马匹中病毒RNA脱落的持续时间和抗体反应的开始时间。
Application of real-time PCR and ELISA assays for equine influenza virus to determine the duration of viral RNA shedding and onset of antibody response in naturally infected horses.
作者信息
Read A J, Finlaison D S, Gu X, Davis R J, Arzey K E, Kirkland P D
机构信息
Virology Laboratory, Elizabeth Macarthur Agriculture Institute, Menangle, NSW 2568, Australia.
出版信息
Aust Vet J. 2011 Jul;89 Suppl 1:42-3. doi: 10.1111/j.1751-0813.2011.00740.x.
Abstract
During the equine influenza (EI) outbreak, two assays were used in parallel to diagnose the disease, to demonstrate freedom from infection in disease control zones and ultimately to demonstrate that EI virus had been eliminated from the Australian horse population. A longitudinal study of a population of naturally infected horses was established to determine the performance characteristics of these assays.
摘要
在马流感（EI）疫情期间，同时使用了两种检测方法来诊断疾病，证明疾病控制区没有感染，并最终证明EI病毒已从澳大利亚马群中消除。对一群自然感染马进行了纵向研究，以确定这些检测方法的性能特征。
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