Graduate School of Natural Science and Technology, Okayama University, 1-1-1 Tsushima-naka, Okayama 700-8530, Japan.
Plant Cell Environ. 2011 Nov;34(11):1900-6. doi: 10.1111/j.1365-3040.2011.02385.x. Epub 2011 Jul 25.
Isothiocyanates (ITCs) are degradation products of glucosinolates in crucifer plants and have repellent effect on insects, pathogens and herbivores. In this study, we report that exogenously applied allyl isothiocyanate (AITC) induced stomatal closure in Arabidopsis via production of reactive oxygen species (ROS) and nitric oxide (NO), and elevation of cytosolic Ca(2+) . AITC-induced stomatal closures were partially inhibited by an inhibitor of NADPH oxidase and completely inhibited by glutathione monoethyl ester (GSHmee). AITC-induced stomatal closure and ROS production were examined in abscisic acid (ABA) deficient mutant aba2-2 and methyl jasmonate (MeJA)-deficient mutant aos to elucidate involvement of endogenous ABA and MeJA. Genetic evidences have demonstrated that AITC-induced stomatal closure required MeJA priming but not ABA priming. These results raise the possibility that crucifer plants produce ITCs to induce stomatal closure, leading to suppression of water loss and invasion of fungi through stomata.
异硫氰酸酯（ITCs）是十字花科植物中硫代葡萄糖苷的降解产物，对昆虫、病原体和草食动物具有驱避作用。在这项研究中，我们报告说，外源施加的丙烯基异硫氰酸酯（AITC）通过产生活性氧（ROS）和一氧化氮（NO）以及升高细胞质 Ca 2+来诱导拟南芥的气孔关闭。NADPH 氧化酶抑制剂部分抑制 AITC 诱导的气孔关闭，而谷胱甘肽单乙酯（GSHmee）完全抑制。在脱落酸（ABA）缺陷突变体 aba2-2 和茉莉酸甲酯（MeJA）缺陷突变体 aos 中检查了 AITC 诱导的气孔关闭和 ROS 产生，以阐明内源性 ABA 和 MeJA 的参与。遗传证据表明，AITC 诱导的气孔关闭需要 MeJA 引发，但不需要 ABA 引发。这些结果提出了这样一种可能性，即十字花科植物产生 ITCs 以诱导气孔关闭，从而抑制水分流失和真菌通过气孔的入侵。
