Research Department of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, UK.
FEMS Microbiol Ecol. 2011 Nov;78(2):349-54. doi: 10.1111/j.1574-6941.2011.01166.x. Epub 2011 Jul 29.
The human oral cavity is host to a complex microbial community estimated to comprise >700 bacterial species, of which at least half are thought to be not yet cultivable in vitro. To investigate the plasmids present in this community, we used a transposon-aided capture system, which allowed the isolation of plasmids from human oral supra- and subgingival plaque samples. Thirty-two novel plasmids and a circular molecule that could be an integrase-generated circular intermediate were isolated.
人类口腔是一个复杂的微生物群落的宿主，据估计该群落包含超过 700 种细菌，其中至少有一半被认为是目前还不能在体外培养的。为了研究该群落中存在的质粒，我们使用了转座子辅助捕获系统，该系统允许从人类口腔龈上和龈下菌斑样本中分离质粒。我们共分离到 32 种新型质粒和一种可能是整合酶产生的环形中间产物的环形分子。
