[从疱疹性角膜炎患者分离出的单纯疱疹病毒对抗病毒药物的体外敏感性]

[In vitro sensitivity to antiviral agents of herpes simplex viruses isolated from patients with herpetic keratitis].

作者信息

Mori Y, Inoue Y, Shimomura Y, Manabe R

机构信息

Department of Ophthalmology, Osaka University Medical School, Japan.

出版信息

Nippon Ganka Gakkai Zasshi. 1990 May;94(5):484-7.

PMID:2171305

Abstract

Thirty-five clinical isolates of herpes simplex virus type 1 (HSV-1) from 34 patients (35 eyes) with herpetic keratitis were examined in vitro for 5-iodo-2'-deoxyuridine (IDU) and acyclovir (ACV) sensitivity. In addition, the effect of clinical treatment with these two drugs in herpetic keratitis was also investigated. The viral effective dose50 (ED50) was defined as the concentration that inhibited the plaque count by 50% compared to the count of the no drug controls. The viral ED50 of IDU ranged from 0.073 to 0.77 micrograms/ml (0.33 +/- 0.16; Mean +/- SD) and that of ACV from 0.0032 to 0.33 micrograms/ml (0.13 +/- 0.11). No virus with markedly diminished sensitivity to IDU and ACV was found. These results suggest that all HSV-1 strains isolated from patients have good sensitivity in vitro to antiviral agents.

摘要

对34例（35只眼）疱疹性角膜炎患者的35株单纯疱疹病毒1型（HSV-1）临床分离株进行了5-碘-2'-脱氧尿苷（IDU）和阿昔洛韦（ACV）体外敏感性检测。此外，还研究了这两种药物对疱疹性角膜炎的临床治疗效果。病毒半数有效剂量（ED50）定义为与无药物对照计数相比使蚀斑计数抑制50%的浓度。IDU的病毒ED50范围为0.073至0.77微克/毫升（0.33±0.16；平均值±标准差），ACV的病毒ED50范围为0.0032至0.33微克/毫升（0.13±0.11）。未发现对IDU和ACV敏感性明显降低的病毒。这些结果表明，从患者分离出的所有HSV-1毒株在体外对抗病毒药物具有良好的敏感性。