淀粉样纤维的沉降速度分析

Sedimentation velocity analysis of amyloid fibrils.

作者信息

Pham Chi Le Lan, Mok Yee-Foong, Howlett Geoffrey J

机构信息

Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia.

出版信息

Methods Mol Biol. 2011;752:179-96. doi: 10.1007/978-1-60327-223-0_12.

DOI:10.1007/978-1-60327-223-0_12

PMID:21713638

Abstract

Analytical ultracentrifugation is a classical technique used to study the solution behavior of proteins. Experimentally determined sedimentation coefficients provide information regarding the size, shape, and interactions of biological macromolecules. Sedimentation velocity methods have been used to characterize the different aggregation states of amyloid oligomers and fibrils. This chapter first describes the theoretical background for sedimentation velocity analysis. It then provides experimental protocols for sedimentation velocity experiments using the analytical ultracentrifuge. Finally, this chapter describes the procedure used to analyze sedimentation velocity data to obtain the size distribution of amyloid fibrils and their oligomeric precursors.

摘要

分析超速离心是一种用于研究蛋白质溶液行为的经典技术。实验测定的沉降系数提供了有关生物大分子的大小、形状和相互作用的信息。沉降速度法已被用于表征淀粉样寡聚体和原纤维的不同聚集状态。本章首先描述沉降速度分析的理论背景。然后提供使用分析超速离心机进行沉降速度实验的实验方案。最后，本章描述了用于分析沉降速度数据以获得淀粉样原纤维及其寡聚前体的大小分布的程序。