Howlett Geoffrey J, Minton Allen P, Rivas Germán
Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Victoria, Australia.
Curr Opin Chem Biol. 2006 Oct;10(5):430-6. doi: 10.1016/j.cbpa.2006.08.017. Epub 2006 Aug 28.
Analytical ultracentrifugation remains pre-eminent among the methods used to study the interactions of macromolecules under physiological conditions. Recent developments in analytical procedures allow the high resolving power of sedimentation velocity methods to be coupled to sedimentation equilibrium approaches and applied to both static and dynamic associations. Improvements in global modeling based on numerical solutions of the Lamm equation have generated new sedimentation velocity applications with an emphasis on data interpretation using sedimentation coefficient or molar mass distributions. Procedures based on the use of multiple optical signals from absorption and interference optics for the analysis of the sedimentation velocity and equilibrium behavior of more complex interactions have now been developed. New applications of tracer sedimentation equilibrium experiments and the development of a fluorescence optical system for the analytical ultracentrifuge extend the accessible concentration range over several orders of magnitude and, coupled with the new analytical procedures, provide powerful new tools for studies of both weak and strong macromolecular interactions in solution.
在用于研究生理条件下大分子相互作用的方法中，分析超速离心法仍然占据着主导地位。分析程序的最新进展使得沉降速度法的高分辨率能够与沉降平衡方法相结合，并应用于静态和动态缔合。基于Lamm方程数值解的全局建模的改进产生了新的沉降速度应用，重点是使用沉降系数或摩尔质量分布进行数据解释。现在已经开发出基于使用来自吸收和干涉光学的多个光学信号来分析更复杂相互作用的沉降速度和平衡行为的程序。示踪沉降平衡实验的新应用以及用于分析超速离心机的荧光光学系统的开发将可及浓度范围扩展了几个数量级，并且与新的分析程序相结合，为研究溶液中弱和强的大分子相互作用提供了强大的新工具。
