Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Hamdard University, New Delhi 110062, India.
Nat Prod Res. 2011 Oct;25(17):1666-70. doi: 10.1080/14786419.2010.529446. Epub 2011 Jun 30.
This study was undertaken to quantify psoralen and daidzein by high-performance thin layer chromatography (HPTLC). The methanolic extract of 10 mg mL(-1) concentration solution was prepared for HPTLC quantification of psoralen and daidzein. HPTLC aluminium-backed plates coated with 0.2 mm layers of silica gel 60 F(254) were used as the stationary phase. The working standard solution of psoralen and daidzein was applied along with the test sample solution by means of Camag Linomat IV sample applicator. R (f) values of psoralen and daidzein were found to be 0.60 and 0.88, whilst as their percentage values in methanolic extract were found to be 3.02% and 5.64% (w/w), respectively. A simple quantitative estimation method of psoralen and daidzein by HPTLC is reported that can be used for the quality control of marketed preparations containing Ficus carica. However, further study is warranted to isolate and quantify active constituents present in the leaves of F. carica by sophisticated techniques.
本研究采用高效薄层色谱法（HPTLC）定量测定补骨脂素和大豆苷元。以 10mg/mL 浓度的甲醇提取物制备补骨脂素和大豆苷元的 HPTLC 定量分析。使用涂有 0.2 毫米厚硅胶 60 F(254)的 HPTLC 铝基板作为固定相。通过 Camag Linomat IV 点样器将工作标准溶液和测试样品溶液一起施加到板上。补骨脂素和大豆苷元的 R(f) 值分别为 0.60 和 0.88，而它们在甲醇提取物中的百分含量分别为 3.02%和 5.64%（w/w）。报道了一种用 HPTLC 定量测定补骨脂素和大豆苷元的简单定量方法，可用于控制含有无花果的市售制剂的质量。然而，需要进一步研究以通过复杂技术分离和定量无花果叶中的活性成分。
