Institute for Chemistry and Biochemistry, Freie Universität Berlin, Thielallee 63, 14195 Berlin, Germany.
Eur J Cell Biol. 2012 Apr;91(4):287-93. doi: 10.1016/j.ejcb.2011.05.004. Epub 2011 Jun 28.
An immense number of cellular processes are initiated by cell surface serine/threonine kinase receptors belonging to the TGF-β/BMP family. Subsequent downstream signalling cascades, as well as their crosstalk results in enormous specificity in terms of phenotypic outcome, e.g. proliferation, differentiation, migration or apoptosis. Such signalling diversity is achieved by the ability of receptors to interact with distinct proteins in a spatio-temporal manner. Following the cloning of the TGF-β/BMP receptors a variety of different technologies were applied to identify such interacting proteins. Here we present a comprehensive survey of known interactome analyses, including our own data, on these receptors and discuss advantages and disadvantages of the applied technologies.
大量的细胞过程是由属于 TGF-β/BMP 家族的细胞表面丝氨酸/苏氨酸激酶受体启动的。随后的下游信号级联反应及其串扰导致表型结果（例如增殖、分化、迁移或凋亡）具有巨大的特异性。这种信号多样性是通过受体以时空方式与不同蛋白质相互作用的能力来实现的。在 TGF-β/BMP 受体被克隆之后，应用了多种不同的技术来鉴定这些相互作用蛋白。在这里，我们对这些受体的已知互作组分析进行了全面的综述，包括我们自己的数据，并讨论了所应用技术的优缺点。
