Gholami-Parizad Elaheh, Taherikalani Morovat, Mozaffar-Sabet Noor-Amir, Asmar Mahdi, Gholami-Parizad Skandar, Khosravi Afra, Emaneini Mohammad, Asadollahi Parisa
Department of Microbiology, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
Acta Microbiol Immunol Hung. 2011 Jun;58(2):105-12. doi: 10.1556/AMicr.58.2011.2.3.
Hepatitis B virus (HBV) transmission via blood and other body fluids from infected individuals to healthy people has been largely demonstrated. However, in the current literature, there is little information available on the potential role of cerumen in HBV transmission. Cerumen and blood were collected from 70 patients infected with HBV and 70 volunteer healthy people were selected as the control group, and the samples were evaluated by ELISA and Real-time PCR. All the patients proved positive for HBsAg and anti HBc total. Sixty-one of the 70 cerumen samples of cases (82.1%) and 5 (7%) of controls were positive for HBV DNA with ranges from 1.53 × 102 to 2.9 × 108 and 1.3 × 102-2.6 × 105/ml, respectively. In three patients, the level of HBV DNA in cerumen was higher than that in the serums. The patients who were positive for HBeAg showed a higher rate of HBVDNA in the serum and cerumen.The results of this study showed the level of HBV DNA as a probably indicator of high risk transmission factor, which was present in the cerumen of chronic hepatitis B patients in west of Iran.
乙肝病毒（HBV）通过感染个体的血液和其他体液传播给健康人的情况已得到充分证实。然而，在当前文献中，关于耳垢在HBV传播中的潜在作用的信息很少。从70例HBV感染患者中采集耳垢和血液，并选择70名健康志愿者作为对照组，通过酶联免疫吸附测定（ELISA）和实时聚合酶链反应（Real-time PCR）对样本进行评估。所有患者的乙肝表面抗原（HBsAg）和总乙肝核心抗体（anti HBc total）均呈阳性。70例患者的耳垢样本中有61例（82.1%）HBV DNA呈阳性，对照组有5例（7%）呈阳性，其含量范围分别为1.53×10²至2.9×10⁸以及1.3×10²至2.6×10⁵/ml。在3例患者中，耳垢中的HBV DNA水平高于血清中的水平。乙肝e抗原（HBeAg）呈阳性的患者血清和耳垢中HBV DNA的检出率更高。本研究结果表明，HBV DNA水平可能是高风险传播因素的一个指标，在伊朗西部慢性乙型肝炎患者的耳垢中存在。
