Gholami Parizad Elaheh, Gholami Parizad Eskandar, Khosravi Afra, Amraei Mansour, Valizadeh Azar, Davoudian Abdoullah
Clinical Microbiology Research Centre, Ilam University of Medical Sciences, Ilam, IR Iran.
Public Health Department, Psychosocial Injuries Prevention Research Centre, Ilam University of Medical Sciences, Ilam, IR Iran.
Hepat Mon. 2016 Apr 27;16(5):e30385. doi: 10.5812/hepatmon.30385. eCollection 2016 May.
Hepatitis B is a disease that is prevalent worldwide and is responsible for 10% of the deaths that occur every year. The virus persists in 5% of infected adults and 90% of infected children and can cause chronic hepatitis. In addition to blood, the virus may also be present in other secretions. Transmission through saliva, sexual fluids, and urine has also been confirmed.
The main aim of this study was to compare viral DNA copies in the serum, cerumen, and saliva of patients with HBeAg levels in their sera.
This was a cross-sectional study and subjects were selected by non-randomized methods. Serum, cerumen, and saliva samples were collected from 50 patients who were diagnosed with chronic hepatitis B about a year prior to the study. Enzyme-linked immunosorbent assay (ELISA) was performed to determine the presence of HBsAg and HBeAg in the gathered specimens. Viral DNA was extracted from specimens by using a Qiagen kit. The number of viral DNA copies was determined using a real-time polymerase chain reaction (PCR) assay. The study was performed in Ilam province in western Iran.
Twenty-eight percent of the patients were HBeAg positive. The average number of viral copies in serum, cerumen, and saliva was higher in women than in men, and a significant correlation was observed between the gender and average viral copies. However, no significant correlation was observed between viral copies present in the serum and cerumen with the age and gender of patients. In addition, no correlation was observed between serum HBeAg and viral copies present in serum, cerumen, and saliva. The correlation analysis confirmed a direct and definite correlation between viral DNA loads in the patients' serum and cerumen.
A significant direct correlation was observed between the viral DNA copies present in patients' cerumen and serum. However, the correlation between saliva viral load with serum and cerumen viral load was very low and inverse. These findings suggest that the presence of the hepatitis B virus (HBV) in non-invasive specimens (such as cerumen and saliva) should also be evaluated when monitoring patients to determine the course of infection and disease.
乙型肝炎是一种在全球范围内流行的疾病，每年导致10%的死亡。该病毒在5%的成年感染者和90%的儿童感染者体内持续存在，并可导致慢性肝炎。除血液外，该病毒也可能存在于其他分泌物中。通过唾液、性液和尿液传播也已得到证实。
本研究的主要目的是比较血清中HBeAg水平不同的患者血清、耵聍和唾液中的病毒DNA拷贝数。
这是一项横断面研究，采用非随机方法选择研究对象。从研究前约一年被诊断为慢性乙型肝炎的50名患者中采集血清、耵聍和唾液样本。采用酶联免疫吸附测定（ELISA）法检测采集标本中HBsAg和HBeAg的存在情况。使用Qiagen试剂盒从标本中提取病毒DNA。采用实时聚合酶链反应（PCR）测定法确定病毒DNA拷贝数。该研究在伊朗西部的伊拉姆省进行。
28%的患者HBeAg呈阳性。女性血清、耵聍和唾液中的病毒拷贝平均数高于男性，且性别与平均病毒拷贝数之间存在显著相关性。然而，血清和耵聍中的病毒拷贝数与患者的年龄和性别之间未观察到显著相关性。此外，血清HBeAg与血清、耵聍和唾液中的病毒拷贝数之间也未观察到相关性。相关性分析证实患者血清和耵聍中的病毒DNA载量之间存在直接且明确的相关性。
观察到患者耵聍和血清中的病毒DNA拷贝数之间存在显著的直接相关性。然而，唾液病毒载量与血清和耵聍病毒载量之间的相关性非常低且呈负相关。这些发现表明，在监测患者以确定感染和疾病进程时，也应评估非侵入性标本（如耵聍和唾液）中乙型肝炎病毒（HBV）的存在情况。
