Albery W J, Cass A E, Mangold B P, Shu Z X
Department of Chemistry, Imperial College, London, UK.
Biosens Bioelectron. 1990;5(5):397-413. doi: 10.1016/0956-5663(90)80018-9.
It is shown that an inhibited enzyme electrode, using cytochrome oxidase, will respond to H2S, HCN and azide ion. For all three inhibitors the kinetics of the inhibiton and recovery processes have been analysed using the theoretical model presented previously (Albery et al., 1990a). Rearrangement of the differential equation describing inhibition and the development of the necessary software has enabled us to obtain values of the concentration of inhibitor in a matter of seconds after exposure of the sensor. The sensor will measure concentrations of H2S down to 1 ppm in the gas phase and concentrations of HCN and azide ion down to 0.4 mumol dm-3 in the solution phase.
结果表明，一种使用细胞色素氧化酶的抑制型酶电极会对硫化氢、氰化氢和叠氮离子产生响应。对于所有这三种抑制剂，已使用先前提出的理论模型（阿尔贝里等人，1990a）分析了抑制和恢复过程的动力学。对描述抑制作用的微分方程进行重新整理以及开发必要的软件，使我们能够在传感器暴露后几秒钟内获得抑制剂浓度值。该传感器能够测量气相中低至1 ppm的硫化氢浓度以及溶液相中低至0.4 μmol dm⁻³的氰化氢和叠氮离子浓度。
