Albery W J, Cass A E, Shu Z X
Department of Chemistry, Imperial College, London, UK.
Biosens Bioelectron. 1990;5(5):367-78. doi: 10.1016/0956-5663(90)80016-7.
A theoretical model is developed for an electrochemical sensor for toxic substances which works by measuring the inhibition of the enzyme activity. The enzyme is assumed to follow Michaelis-Menten kinetics and the diffusion kinetic equation describing the concentration profile of the enzyme's substrate in the electrolyte layer between the electrode and the membrane covering the electrode is solved. A complete set of analytical solutions is found which corresponds to a number of different rate limiting processes. The set of solutions is described in a case diagram. The use of cytochrome oxidase in particular is discussed.
开发了一种用于检测有毒物质的电化学传感器的理论模型，该传感器通过测量酶活性的抑制作用来工作。假设该酶遵循米氏动力学，并求解了描述电极与覆盖电极的膜之间电解质层中酶底物浓度分布的扩散动力学方程。找到了一组完整的解析解，它们对应于许多不同的速率限制过程。该组解在一个案例图中进行了描述。特别讨论了细胞色素氧化酶的使用。
