Interdisciplinary Programs of Bioengineering, Seoul National University, Seoul, Korea.
J Microbiol Biotechnol. 2011 Jun;21(6):613-6.
Streptomyces venezuelae YJ028, bearing a deletion of the entire biosynthetic gene cluster encoding the pikromycin polyketide synthases and desosamine biosynthetic enzymes, was used as a bioconversion system for combinatorial biosynthesis of glycosylated derivatives of tylosin. Two engineered deoxysugar biosynthetic pathways for the biosynthesis of TDP-3-O-demethyl-D-chalcose or TDP-Lrhamnose in conjunction with the glycosyltransferaseauxiliary protein pair DesVII/DesVIII were expressed in a S. venezuelae YJ028 mutant strain. Supplementation of each mutant strain capable of producing TDP-3-O-demethyl- D-chalcose or TDP-L-rhamnose with tylosin aglycone tylactone resulted in the production of the 3-O-demethyl- D-chalcose, D-quinovose, or L-rhamnose-glycosylated tylactone.
委内瑞拉链霉菌 YJ028 缺失了整个生物合成基因簇，该基因簇编码的是比基尼霉素聚酮合酶和去氧氨基糖生物合成酶，被用作泰乐菌素糖基化衍生物组合生物合成的生物转化系统。两个工程化的脱氧糖生物合成途径用于 TDP-3-O-去甲基-D-赤藓糖或 TDP-L-鼠李糖的生物合成，与糖基转移酶辅助蛋白对 DesVII/DesVIII 一起在委内瑞拉链霉菌 YJ028 突变株中表达。将能够产生 TDP-3-O-去甲基-D-赤藓糖或 TDP-L-鼠李糖的每个突变株与泰乐菌素苷元泰乐内酯一起补充，导致 3-O-去甲基-D-赤藓糖、D-奎诺糖或 L-鼠李糖糖基化的泰乐内酯的产生。
