Cho Keng-Chi, Lien Chi-Hsiang, Lin Chun-Yu, Chang Chia-Yuan, Huang Lynn L H, Campagnola Paul J, Dong Chen Yuan, Chen Shean-Jen
Institute of Electro-Optical Science and Engineering, National Cheng Kung University, Tainan, Taiwan.
Opt Express. 2011 Jun 6;19(12):11732-9. doi: 10.1364/OE.19.011732.
In this study, the intensity of two-photon excited fluorescence (TPEF) of xanthene dye, Rose Bengal (RB), was significantly enhanced via bovine serum albumin (BSA) microstructures fabricated by the two-photon crosslinking (TPC) technique. The RB was utilized as the photoactivator in the TPC processing and the enhanced TPEF intensity correlates with the concentration of fabricated crosslinked BSA microstructures via the power control and pulse selection of the employed femtosecond laser. As a result, fabrication of three-dimensional BSA microstructures can be simultaneously monitored by the use of TPEF intensity. The crosslinked BSA microstructures synthesized may be used as an ordered biomaterial for fluorescence enhancement.
在本研究中，通过双光子交联（TPC）技术制备的牛血清白蛋白（BSA）微结构显著增强了呫吨染料孟加拉玫瑰红（RB）的双光子激发荧光（TPEF）强度。RB被用作TPC过程中的光活化剂，通过所采用的飞秒激光的功率控制和脉冲选择，增强的TPEF强度与制备的交联BSA微结构的浓度相关。因此，利用TPEF强度可以同时监测三维BSA微结构的制备。合成的交联BSA微结构可用作增强荧光的有序生物材料。
