Lin Chun-Yu, Lien Chi-Hsiang, Cho Keng-Chi, Chang Chia-Yuan, Chang Nan-Shan, Campagnola Paul J, Dong Chen Yuan, Chen Shean-Jen
Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan.
Opt Express. 2012 Jun 18;20(13):13669-76. doi: 10.1364/OE.20.013669.
The two-photon excited fluorescence (TPEF) increments of two dyes via bovine serum albumin (BSA) microstructures fabricated by the two-photon crosslinking technique were investigated. One is Rose Bengal (RB) with a high non-radiative decay rate, while the other is Eosin Y with a low non-radiative decay rate. Experimental results demonstrate that the quantum yield and lifetime of RB are both augmented via crosslinked BSA microstructures. Compared with theoretical analysis, this result indicates that the non-radiative decay rate of RB is decreased; hence, the quenched effect induced by BSA solution is suppressed. However, the fluorescence lifetime of Eosin Y is acutely abated despite the augmented quantum yield for the two-photon crosslinking processing from BSA solution. This result deduces that the radiative decay rate increased. Furthermore, the increased TPEF intensity and lifetime of RB correlated with the concentration of fabricated crosslinked BSA microstructures through pulse selection of the employed femtosecond laser is demonstrated and capable of developing a zone-plate-like BSA microstructure.
研究了通过双光子交联技术制备的牛血清白蛋白（BSA）微结构对两种染料的双光子激发荧光（TPEF）增量。一种是具有高非辐射衰减率的孟加拉玫瑰红（RB），另一种是具有低非辐射衰减率的曙红Y。实验结果表明，通过交联的BSA微结构，RB的量子产率和寿命均得到提高。与理论分析相比，该结果表明RB的非辐射衰减率降低；因此，BSA溶液引起的猝灭效应得到抑制。然而，尽管双光子交联处理的BSA溶液使曙红Y的量子产率提高，但其荧光寿命却急剧缩短。该结果推断辐射衰减率增加。此外，通过所采用的飞秒激光的脉冲选择，证明了RB的TPEF强度和寿命的增加与制备的交联BSA微结构的浓度相关，并且能够形成类似波带片的BSA微结构。
