Department of Surgical Medicine, Erlin Branch of Changhua Christian Hospital, Changhua 526, Taiwan.
Evid Based Complement Alternat Med. 2011;2011:841564. doi: 10.1155/2011/841564. Epub 2011 May 24.
Tanshinone IIA (Tan IIA) and sodium tanshinone IIA sulfonate (STS) were found to have protective effects on cardiomyocyte against adriamycin-induced damage and may be used clinically. It is unclear whether the supplementation of STS or Tan IIA would affect the anticancer activity of anthracycline. To evaluate the effect of Tan IIA or STS on the anticancer of epirubicin, the cell viability, apoptosis, Akt expression, and uptake of epirubicin after supplementation of Tan IIA or STS in the epirubicin-treated BT-20 cells were measured and compared. Tan IIA inhibited BT-20 cell growth and induced apoptosis in a time- and dose-dependent manner. When Tan IIA was used with epirubicin, an increase of BT-20 cells apoptosis was accompanied by the decreasing phosphorylation of Akt. STS had no effect on the cell viability of BT-20 cells. However, when used with epirubicin, STS decreased the epirubicin-induced cytotoxicity and apoptosis in BT-20 cells. The antagonistic effect of STS on epirubicin-induced cytotoxicity in BT-20 cells occurred concomitantly with the reduced epirubicin uptake and the increased phosphorylation of Akt. STS decreased the uptake of epirubicin in BT-20 cells and blocked epirubicin-induced apoptosis through activation of Akt.
丹参酮 IIA（Tan IIA）和丹参酮 IIA 磺酸钠（STS）已被发现对阿霉素诱导的心肌细胞损伤具有保护作用，可能在临床上使用。目前尚不清楚 STS 或 Tan IIA 的补充是否会影响蒽环类药物的抗癌活性。为了评估 Tan IIA 或 STS 对表阿霉素抗癌活性的影响，测量并比较了在表阿霉素处理的 BT-20 细胞中补充 Tan IIA 或 STS 后细胞活力、细胞凋亡、Akt 表达和表阿霉素摄取的变化。Tan IIA 呈时间和剂量依赖性抑制 BT-20 细胞生长并诱导细胞凋亡。当 Tan IIA 与表阿霉素联合使用时，BT-20 细胞凋亡的增加伴随着 Akt 的磷酸化减少。STS 对 BT-20 细胞的细胞活力没有影响。然而，当与表阿霉素联合使用时，STS 降低了 BT-20 细胞中表阿霉素诱导的细胞毒性和细胞凋亡。STS 对 BT-20 细胞中表阿霉素诱导的细胞毒性的拮抗作用与表阿霉素摄取减少和 Akt 磷酸化增加同时发生。STS 降低了 BT-20 细胞中表阿霉素的摄取，并通过激活 Akt 阻断表阿霉素诱导的细胞凋亡。
