Programa de Pós-Graduação em Biologia Geral e Aplicada, Universidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatu, São Paulo, Brazil.
J Appl Toxicol. 2012 Oct;32(10):810-4. doi: 10.1002/jat.1701. Epub 2011 Jun 30.
Nandrolone is an androgenic-anabolic steroid (AAS) with diverse medical applications but taken indiscriminately by some to rapidly increase muscle mass. The aim of this study was to evaluate the genotoxic and clastogenic potential of nandrolone (deca-durabolin®) in vivo in different cells of mice, using the comet assay and micronucleus test, respectively. The animals received subcutaneous injection of the three doses of the steroid (1.0, 2.5 and 5.0 mg kg⁻¹ body weight). Cytotoxicity was assessed by scoring 200 consecutive total polychromatic (PCE) and normochromatic (NCE) erythrocytes (PCE-NCE ratio). The results showed a significant dose-related increase in the frequency of DNA damage in leukocytes, liver, bone marrow, brain and testicle cells at the three tested doses and a significant increase of the micronucleated polychromatic erythrocytes at all tested doses. Under our experimental conditions, the nandrolone steroid hormone showed genotoxic and clastogenic effects when administered subcutaneously to mice.
诺龙是一种具有多种医学应用的雄激素-合成代谢类固醇（AAS），但有些人不加选择地使用它来快速增加肌肉质量。本研究旨在使用彗星试验和微核试验分别评估诺龙（癸酸诺龙®）在体内对不同细胞的遗传毒性和断裂剂潜力。动物接受皮下注射三种剂量的类固醇（1.0、2.5 和 5.0mg/kg 体重）。通过评分 200 个连续的总多色性（PCE）和正常色性（NCE）红细胞（PCE-NCE 比值）来评估细胞毒性。结果显示，在三种测试剂量下，白细胞、肝脏、骨髓、大脑和睾丸细胞中的 DNA 损伤频率以及所有测试剂量下的多色性微核红细胞数量均显著增加。在我们的实验条件下，诺龙类固醇激素在给小鼠皮下注射时表现出遗传毒性和断裂剂作用。
