Universidade Estadual Paulista - UNESP - Instituto de Biociências, Programa de Pós-Graduação em Biologia Geral e Aplicada, Botucatu, SP, Brazil.
J Ethnopharmacol. 2012 Jul 13;142(2):362-6. doi: 10.1016/j.jep.2012.04.045. Epub 2012 May 17.
Garcinia achachairu Rusby (Clusiaceae) is popularly known as "achachairu", and is used in Bolivian folk medicine for its healing, digestive, and laxative properties, and in the treatment of gastritis, rheumatism and inflammation. Despite its widespread therapeutic use, there is a lack of data regarding its in vivo genotoxic effects. Therefore, in this study, we used the comet assay and the micronucleus test, respectively, to evaluate the possible genotoxic and clastogenic effects of Garcinia achachairu seed extract (GAE) on different cells of mice.
The GAE was administered by oral gavage at doses of 500, 1000 and 2000 mg/kg. For the analysis, the comet assay was performed on the leukocytes (collected 4 and 24 h after treatment), liver, bone marrow and testicular cells (collected 24 h after treatment), and the micronucleus test (MN) on bone marrow cells. Cytotoxicity was assessed by scoring 200 consecutive polychromatic (PCE) and normochromatic (NCE) erythrocytes (PCE/NCE ratio).
The results showed that GAE did not induce significant DNA damage in leukocytes (4 h and 24 h samples), liver, bone marrow and testicular cells (24 h samples). GAE also did not show any significant increase in micronucleated polychromatic erythrocytes (MNPCEs) at the three tested doses. The PCE/NCE ratio indicated no cytotoxicity. Under our experimental conditions, the data obtained suggest that a single oral administration of G. achachairu extract does not cause genotoxicity and clastogenicity in different cells of mice.
藤黄科的 Achachairu（Garcinia achachairu Rusby），俗称“achachairu”，在玻利维亚民间医学中被用于治疗、消化和通便，以及治疗胃炎、风湿和炎症。尽管其具有广泛的治疗用途，但缺乏关于其体内遗传毒性的数据。因此，在这项研究中，我们分别使用彗星试验和微核试验评估 Garcinia achachairu 种子提取物（GAE）对不同小鼠细胞的可能遗传毒性和断裂作用。
GAE 通过口服灌胃给予 500、1000 和 2000 mg/kg 剂量。用于分析，在处理后 4 和 24 小时对白细胞（收集）、肝、骨髓和睾丸细胞进行彗星试验，在骨髓细胞中进行微核试验（MN）。通过评分 200 个连续的多色性（PCE）和正常色性（NCE）红细胞（PCE/NCE 比）评估细胞毒性。
结果表明，GAE 未在白细胞（4 小时和 24 小时样本）、肝、骨髓和睾丸细胞（24 小时样本）中引起明显的 DNA 损伤。GAE 在三个测试剂量下也未显示微核多色性红细胞（MNPCEs）的显著增加。PCE/NCE 比表明无细胞毒性。在我们的实验条件下，所得数据表明单次口服 G. achachairu 提取物不会导致不同小鼠细胞的遗传毒性和断裂作用。
