肝病患者血清中苹果酸脱氢酶及其线粒体同工酶与天冬氨酸氨基转移酶及其线粒体同工酶相比的临床实用性。

Clinical usefulness of malate dehydrogenase and its mitochondrial isoenzyme in comparison with aspartate aminotransferase and its mitochondrial isoenzyme in sera of patients with liver disease.

作者信息

Kawai M, Hosaki S

机构信息

Third Department of Internal Medicine, Faculty of Medicine, Tokyo Medical and Dental University, Japan.

出版信息

Clin Biochem. 1990 Aug;23(4):327-34. doi: 10.1016/0009-9120(90)80064-p.

DOI:10.1016/0009-9120(90)80064-p

PMID:2171815

Abstract

The activities of serum malate dehydrogenase (MDH) and its mitochondrial isoenzyme (MDHm) were studied in sera of patients with liver disease. They proved to be more useful than those of aspartate aminotransferase (AST) and its mitochondrial isoenzyme for detection of hepatocellular carcinoma and acute circulatory failure, and for estimation of the severity of acute hepatitis. The N/T value measuring system, which is adaptable for autoanalysis and allows simultaneous determination of activities depending on NAD and thionicotinamide adenine dinucleotide (thio-NAD), yields both the total activity of MDH and the N/T value which was correlated significantly with MDHm/MDH (r = 0.748). Assay of MDH and its mitochondrial isoenzyme in association with the N/T value measuring system seems to be more useful and less time consuming for estimation of the severity of liver diseases than that of AST and its mitochondrial isoenzyme.

摘要

对肝病患者血清中的苹果酸脱氢酶（MDH）及其线粒体同工酶（MDHm）的活性进行了研究。结果证明，它们在检测肝细胞癌和急性循环衰竭以及评估急性肝炎的严重程度方面，比天冬氨酸转氨酶（AST）及其线粒体同工酶更有用。N/T值测量系统适用于自动分析，能够同时测定依赖于烟酰胺腺嘌呤二核苷酸（NAD）和硫代烟酰胺腺嘌呤二核苷酸（硫代-NAD）的活性，可得出MDH的总活性以及与MDHm/MDH显著相关的N/T值（r = 0.748）。与AST及其线粒体同工酶相比，联合N/T值测量系统检测MDH及其线粒体同工酶，在评估肝脏疾病严重程度方面似乎更有用且耗时更少。