Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland.
J Appl Microbiol. 2011 Aug;111(2):467-73. doi: 10.1111/j.1365-2672.2011.05067.x. Epub 2011 Jul 1.
The aim of this investigation was to establish the degree of correlation between measurements from culture-dependent microbiological techniques and from next generation sequencing technologies.
Data generated by both techniques were collected from faecal samples from 185 elderly Irish people involved in the ongoing ELDERMET study (http://eldermet.ucc.ie). The results for three groups of intestinal bacteria were compared. Bifidobacterium sp., Lactobacillus sp. and Enterobacteriaceae were enumerated on selective media through culture-dependent techniques, whereas proportions of these bacteria were determined through sequencing technology against the background of other bacteria. The Spearman's rank correlation coefficient determined a good correlation between results from culture-dependent microbiology and culture-independent techniques for all three bacterial groups assessed (correlation coefficients for Bifidobacterium sp., Lactobacillus sp. and Enterobacteriaceae were 0·380, 0·366 and 0·437, respectively).
Correlation between the two methods implies that a single method is capable of profiling intestinal Bifidobacterium, Lactobacillus and Enterobacteriaceae populations. However, both methods have advantages that justify their use in tandem.
This is the first extensive study to compare bacterial counts from culture-dependent microbiological techniques and from next generation sequencing technologies.
本研究旨在确定基于培养的微生物学技术与下一代测序技术测量结果之间的相关性程度。
本研究从正在进行的 ELDERMET 研究（http://eldermet.ucc.ie）中 185 名爱尔兰老年人的粪便样本中收集了两种技术生成的数据。比较了三组肠道细菌的结果。通过基于培养的技术，在选择性培养基上对双歧杆菌属、乳杆菌属和肠杆菌科进行了计数，而通过测序技术在其他细菌背景下确定了这些细菌的比例。Spearman 等级相关系数确定了三种评估细菌组中基于培养的微生物学和非培养技术之间的良好相关性（双歧杆菌属、乳杆菌属和肠杆菌科的相关系数分别为 0.380、0.366 和 0.437）。
两种方法之间的相关性意味着单一方法能够对肠道双歧杆菌、乳杆菌和肠杆菌科种群进行分析。然而，这两种方法都有其优势，证明它们可以串联使用。
这是首次比较基于培养的微生物学技术和下一代测序技术的细菌计数的广泛研究。
