Griffiths William J, Wang Yuqin
Swansea University, Swansea, UK.
Biochim Biophys Acta. 2011 Nov;1811(11):784-99. doi: 10.1016/j.bbalip.2011.05.012. Epub 2011 Jun 12.
Oxysterols are oxygenated forms of cholesterol. This definition can, however, be expanded to include oxygenated derivatives of plant sterols and also of cholesterol precursors. Oxysterols are formed in the first steps of cholesterol metabolism and also from cholesterol by reactive oxygen species. Oxysterols were once thought of as simple intermediates, or side-products, in the conversion of cholesterol to hormonal steroids and bile acids, however, they have subsequently been shown to be biologically active molecules in their own right. In this article we will discuss methods of oxysterol analysis including "classical" gas chromatography-mass spectrometry (GC-MS) methods and more recent liquid chromatography (LC)-MS methods. Our main focus, however, will be on analytical methods based on "charge-tagging" and LC-tandem mass spectrometry (MS/MS or MS(n)) which we have developed over the last decade in our laboratory. Examples will be given of oxysterol analysis in brain, cerebrospinal fluid (CSF) and blood. The advantages and disadvantages of the various methods of oxysterol analysis will be discussed.
氧化甾醇是胆固醇的氧化形式。然而，这个定义可以扩展到包括植物甾醇以及胆固醇前体的氧化衍生物。氧化甾醇在胆固醇代谢的最初步骤中形成，也可由活性氧从胆固醇生成。氧化甾醇曾被认为是胆固醇转化为激素类固醇和胆汁酸过程中的简单中间体或副产物，然而，后来它们被证明本身就是具有生物活性的分子。在本文中，我们将讨论氧化甾醇分析方法，包括“经典”气相色谱 - 质谱联用（GC - MS）方法和更新的液相色谱（LC） - 质谱联用方法。不过，我们的主要重点将是基于“电荷标记”和液相色谱 - 串联质谱（MS/MS或MS(n)）的分析方法，这些方法是我们在过去十年中在实验室开发的。将给出在脑、脑脊液（CSF）和血液中氧化甾醇分析的示例。还将讨论各种氧化甾醇分析方法的优缺点。
