Berrios M, Avilion A A
Department of Pharmacological Sciences, School of Medicine, State University of New York, Stony Brook 11794-8651.
Exp Cell Res. 1990 Nov;191(1):64-70. doi: 10.1016/0014-4827(90)90036-a.
A cell-free preparation obtained from 0- to 5-h-old Drosophila melanogaster embryos induces chromatin decondensation and nuclear formation from demembranated Xenopus sperm. Newly formed nuclei have a peripheral lamina, a double membrane, and structures resembling pore complexes. Indirect immunofluorescence analyses demonstrate the association of Drosophila lamins and DNA topoisomerase II with newly assembled nuclei.
从0至5小时大的黑腹果蝇胚胎中获得的无细胞制剂可诱导去膜爪蟾精子发生染色质解聚和细胞核形成。新形成的细胞核具有外周核纤层、双层膜以及类似核孔复合体的结构。间接免疫荧光分析表明果蝇核纤层蛋白和DNA拓扑异构酶II与新组装的细胞核相关联。
