Yagi Naoto
Japan Synchrotron Radiation Research Institute.
Clin Calcium. 2011 Jul;21(7):1030-5.
Bone researches with synchrotron radiation have now expanded from a simple mineralization measurement to more complicated studies such as strain mapping and tissue engineering. The most common techniques are high resolution X-ray tomography and X-ray diffraction, but the spectroscopic techniques such as fluorescence analysis, infrared FTIR and XAFS are also available. High resolution X-ray tomography has been improved in the time and spatial resolution. In vitro tomographic experiments have become common and been applied to 3D imaging of a live mouse. As a new imaging method, coherent diffraction imaging has been applied to visualize the lacunocanalicular network. As each of these techniques provides unique information and the sample condition depends much on the technique, the choice of the technique is important to achieve a scientific goal.
利用同步辐射进行的骨研究现已从简单的矿化测量扩展到更复杂的研究，如应变映射和组织工程。最常用的技术是高分辨率X射线断层扫描和X射线衍射，但荧光分析、红外傅里叶变换红外光谱（FTIR）和X射线吸收精细结构（XAFS）等光谱技术也可供使用。高分辨率X射线断层扫描在时间和空间分辨率方面都有了改进。体外断层扫描实验已变得很常见，并已应用于活小鼠的三维成像。作为一种新的成像方法，相干衍射成像已被用于可视化骨陷窝小管网络。由于这些技术中的每一种都提供独特的信息，并且样品条件在很大程度上取决于技术，因此选择合适的技术对于实现科学目标很重要。
