Ogiso Motoki, Kimura Ayako, Chikasou Masato, Saito Maki, Takemoto Masami, Watai Masatoshi
Japan Food Research Laboratories, Tama Laboratory, Tokyo.
Shokuhin Eiseigaku Zasshi. 2011;52(3):161-6. doi: 10.3358/shokueishi.52.161.
A highly sensitive analysis method for six aflatoxins (aflatoxin B₁, B₂, G₁, G₂, M₁ and aflatoxicol) in rainbow trout muscle and liver was developed. Aflatoxins (AFs) were extracted with acetonitrile-water (9 : 1), purified on an immunoaffinity column, and subjected to HPLC with fluorescence detection after post-column photochemical derivatization. The recoveries of AFs at 0.05 μg/kg spiking levels were 71.4-82.4% in muscle and 80.1-93.0% in liver, and the repeatability relative standard deviations (RSDr) were 0.87-4.6% in muscle and 2.0-6.2% in liver. Limits of quantitation (LOQs) and limits of detection(LODs)of AFs were estimated to be 0.004-0.029 μg/kg, and 0.002-0.012 μg/kg, respectively.
