Cui J, Shen X, Zhao H, Nagahama Y
Department of Epidemiology and Public Health, National University of Singapore. jianzhou_cui @ nuhs.edu.sg
Cytogenet Genome Res. 2011;134(4):283-94. doi: 10.1159/000329480. Epub 2011 Jun 29.
Genes of the Sox family encode evolutionarily conserved high-mobility group box containing transcription factors, which play key roles in various events of developmental contexts. In this study, we identified 15 sox genes by searching for the high-mobility group domain in the medaka genome and by polymerase chain reaction using primers designed from the results obtained from homology protein alignment. All medaka sox genes except a novel sox gene, Olsox32, are encoded in 5 groups as follows: 4 sox genes in group B; 3 sox genes in group D and F, respectively; 2 sox genes in group C and E, respectively, while no sox genes were found in groups A, G, H, I, and J. The medaka Olsox32 does not fall within any of the previously defined groups A-J. Here we have assigned it to a new group K. Together with the Sox protein sequences of other species, the phylogenetic relationship was analyzed and compared. Our findings point to recent sox gene loss, duplication and divergence occurring during the evolution of tetrapod and teleost lineages. The expression pattern shows that sox genes play a variety of roles in the early embryonic development of medaka.
Sox家族基因编码具有进化保守性的、包含高迁移率族盒结构域的转录因子，这些转录因子在发育过程的各种事件中发挥关键作用。在本研究中，我们通过在青鳉基因组中搜索高迁移率族结构域，并使用根据同源蛋白比对结果设计的引物进行聚合酶链反应，鉴定出了15个sox基因。除了一个新的sox基因Olsox32外，所有青鳉sox基因如下分为5组：B组有4个sox基因；D组和F组分别有3个sox基因；C组和E组分别有2个sox基因，而在A、G、H、I和J组中未发现sox基因。青鳉Olsox32不属于先前定义的A - J组中的任何一组。在此我们将其归为一个新的K组。结合其他物种的Sox蛋白序列，分析并比较了系统发育关系。我们的研究结果表明，在四足动物和硬骨鱼谱系的进化过程中发生了近期的sox基因丢失、复制和分化。表达模式表明，sox基因在青鳉早期胚胎发育中发挥多种作用。
