氧化亚铁硫杆菌核糖体RNA基因启动子的鉴定与结构分析

Identification and structural analysis of a ribosomal RNA gene promoter from Thiobacillus ferrooxidans.

作者信息

Takamiya M, Salazar O, Vargas D, Jedlicki E, Orellana O

机构信息

Departamento de Bioquimica, Facultad de Medicina, Universidad de Chile, Santiago.

出版信息

FEBS Lett. 1990 Oct 15;272(1-2):50-4. doi: 10.1016/0014-5793(90)80446-p.

DOI:10.1016/0014-5793(90)80446-p

PMID:2172018

Abstract

The 5'-terminus of a rRNA operon (rrnT2) from Thiobacillus ferrooxidans was characterized. The rRNA promoters from this microorganism were identified by means of a functional assay in Escherichia coli. DNA sequencing of the promoter region, upstream the 16 S rRNA gene, showed the presence of a consensus sequence for bacterial ribosomal promoters. Other features such as a 'discriminator' sequence, antiterminator elements and an upstream hexanucleotide common to several rRNA operons were also found. Two other putative transcription promoters were also identified.

摘要

对氧化亚铁硫杆菌的一个rRNA操纵子（rrnT2）的5'端进行了表征。通过在大肠杆菌中的功能测定鉴定了该微生物的rRNA启动子。对16S rRNA基因上游启动子区域的DNA测序显示存在细菌核糖体启动子的共有序列。还发现了其他特征，如“鉴别器”序列、抗终止元件以及几个rRNA操纵子共有的上游六核苷酸。还鉴定出另外两个推定的转录启动子。