大肠杆菌rrnG核糖体RNA启动子区域的核苷酸序列。
Nucleotide sequence of the rrnG ribosomal RNA promoter region of Escherichia coli.
作者信息
Shen W F, Squires C, Squires C L
出版信息
Nucleic Acids Res. 1982 May 25;10(10):3303-13. doi: 10.1093/nar/10.10.3303.
Abstract
The primary structure of the promoter region for a ribosomal RNA transcription unit (rrnG) of Escherichia coli K12 has been determined. The sequence was obtained from 1 1.5 kbp EcoRI fragment derived from the hybrid plasmid pLC23-30. This fragment contains 455 bp preceding P1 of the rrnG promoter region and 674 bp of the rrnG 16S RNA gene. The sequence before the rrnG promoter region contains an open reading frame (ORF-BG) followed by a possible hairpin structure that resembles other known transcription terminators. The sequence of the rrnG promoter region is similar but not identical to that of rrnA and rrnB. Several minor differences between the sequences of the 16S RNA genes of rrnG and rrnB were also noted. In addition, sequences were found that could generate special structures involving the promoter regions of rrn loci. Such structures are described and their possible involvement in the regulation of ribosomal RNA synthesis is discussed.
摘要
已确定大肠杆菌K12核糖体RNA转录单位（rrnG）启动子区域的一级结构。该序列取自源自杂交质粒pLC23 - 30的11.5 kbp EcoRI片段。该片段包含rrnG启动子区域P1之前的455 bp以及rrnG 16S RNA基因的674 bp。rrnG启动子区域之前的序列包含一个开放阅读框（ORF - BG），其后是一个类似于其他已知转录终止子的可能发夹结构。rrnG启动子区域的序列与rrnA和rrnB的序列相似但不相同。还注意到rrnG和rrnB的16S RNA基因序列之间的几个微小差异。此外，发现了一些序列，它们可能形成涉及rrn基因座启动子区域的特殊结构。本文描述了这些结构，并讨论了它们可能参与核糖体RNA合成调控的情况。
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