Rosche E, Westhoff P
Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität Düsseldorf, FRG.
FEBS Lett. 1990 Oct 29;273(1-2):116-21. doi: 10.1016/0014-5793(90)81064-u.
We have isolated and characterized cDNA clones encoding the entire precursor for the leafspecific isoform of pyruvate, orthophosphate dikinase (PPDK) from the dicotyledonous C4 plant Flaveria trinervia. The deduced amino acid sequence reveals a high degree of similarity to the corresponding maize protein indicating a common evolutionary basis. However, no significant similarities are apparent upon comparison of the putative transit peptides. The implications of this divergence are discussed with respect to the evolution of PPDK genes.
我们已经从双子叶C4植物三脉黄顶菊(Flaveria trinervia)中分离并鉴定了编码丙酮酸正磷酸双激酶(PPDK)叶片特异性同工型的整个前体的cDNA克隆。推导的氨基酸序列显示与相应的玉米蛋白有高度相似性,表明存在共同的进化基础。然而,在比较推定的转运肽时,没有明显的显著相似性。本文就PPDK基因的进化讨论了这种差异的影响。