Department of Neurosurgery, Shengjing Hospital Affiliated of China Medical University, Shenyang, PR China.
Int J Mol Med. 2011 Nov;28(5):739-44. doi: 10.3892/ijmm.2011.734. Epub 2011 Jun 27.
Calmodulin (CaM) is a multifunctional receptor of intercellular Ca2+, which under different physiological conditions or at different developmental stages plays different roles in different tissues and cells. This study aimed to investigate the involvement of spatial expression and coexistence of CaM and actin in directed differentiation of rat cortical neural stem cells (NSCs) into neurons. Immunohistochemistry, RT-PCR and immunofluorescence dual-labeling technology was conducted to investigate the temporal and spatial pattern of CaM and actin proteins in neuron-oriented cortical NSC differentiation. Confocal laser scanning microscopy (CLSM) was used to observe changes of the coexistence in protein expression. Our results showed that cortical NSCs expressed and showed localized CaM and actin in a well-defined temporal order. In the process of rat neuron-oriented cerebral cortical NSC differentiation, CaM displayed a similar expression pattern with actin in the development of neurons, that is, both proteins extended into neurites following the sprouting and growth of neurons. These results suggest that the temporal and spatial pattern of CaM and actin expression is comparable to the growth of cell processes in differentiating NSCs. Therefore, both CaM and actin may jointly participate in the development and maturation of neurites, and this provides a theoretical basis for further study of the biological features of neuron-oriented NSC differentiation.
钙调蛋白（CaM）是细胞间 Ca2+的多功能受体，在不同的生理条件或不同的发育阶段，在不同的组织和细胞中发挥不同的作用。本研究旨在探讨 CaM 和肌动蛋白在定向分化为神经元的大鼠皮质神经干细胞（NSC）中的空间表达和共存情况。通过免疫组织化学、RT-PCR 和免疫荧光双重标记技术，研究了 CaM 和肌动蛋白蛋白在定向分化为神经元的皮质 NSC 中的时空表达模式。共聚焦激光扫描显微镜（CLSM）用于观察蛋白表达共存的变化。结果表明，皮质 NSCs 以明确的时间顺序表达并显示局部 CaM 和肌动蛋白。在大鼠神经元定向皮质 NSC 分化过程中，CaM 与肌动蛋白在神经元的发育过程中呈现出相似的表达模式，即两种蛋白都随着神经元的突起和生长延伸到神经突中。这些结果表明，CaM 和肌动蛋白的时空表达模式与分化 NSCs 中细胞突起的生长相似。因此，CaM 和肌动蛋白可能共同参与神经突的发育和成熟，为进一步研究神经元定向 NSC 分化的生物学特性提供了理论依据。
