Department of Breast Pathology, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Breast Cancer Prevention and Therapy of the Ministry of Education and Key Laboratory of Cancer Prevention and Therapy of Tianjin, Tianjin 300060, China.
Chin Med J (Engl). 2011 Oct;124(20):3394-8.
Neural stem cells (NSCs) are a self-renewing and multipotent population of the central nervous system (CNS), which are active during development and maintain homeostasis and tissue integrity throughout life. Microglias are an immune cell population resident in the CNS, which have crucial physiological functions in the developing and adult CNS. This study aimed to investigate that whether microglia co-cultured with NSCs could promote astrogliogenesis from NSCs.
Microglia and NSCs were co-cultured in 24-well insert plates. NSCs were plated in the bottom of the well and microglia in the insert. Fluorescent staining, Western blotting and RT-PCR were used to determine the effect of microglia on NSCs differentiation.
Co-culture of microglia and NSCs promoted astrogliogenesis from NSCs. Several key genes, such as Notch 1, Notch 2, Notch 3, Hes 5, and NRSF were downregulated, while the critical genes Id1 and Id2 were upregulated. BMP2 and FGF2 were upregulated.
Microglias act as a regulator of NSCs astrogliogenesis.
神经干细胞（NSCs）是中枢神经系统（CNS）中具有自我更新和多能性的细胞群体，它们在发育过程中活跃，并在整个生命周期中维持内稳态和组织完整性。小胶质细胞是驻留在中枢神经系统中的免疫细胞群体，它们在发育中和成年的中枢神经系统中具有至关重要的生理功能。本研究旨在探讨与小胶质细胞共培养的 NSCs 是否能促进 NSCs 的星形胶质细胞分化。
将小胶质细胞和 NSCs 共培养于 24 孔插板中。将 NSCs 接种到底部孔中，小胶质细胞接种于插板中。通过荧光染色、Western blot 和 RT-PCR 检测小胶质细胞对 NSCs 分化的影响。
小胶质细胞和 NSCs 的共培养促进了 NSCs 的星形胶质细胞分化。一些关键基因，如 Notch1、Notch2、Notch3、Hes5 和 NRSF 下调，而关键基因 Id1 和 Id2 上调。BMP2 和 FGF2 上调。
小胶质细胞作为 NSCs 星形胶质细胞分化的调节剂。
