School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, China.
Biotechnol Lett. 2011 Aug;33(8):1581-5. doi: 10.1007/s10529-011-0616-6. Epub 2011 Apr 12.
Resin-based, in situ product removal (ISPR) was used to increase production of ε-poly-L-lysine (PL) by Streptomyces sp. GIM8. D152 resin was selected over Amberlite IRC-50, Amberlite IRC-76 and Amberlite IR-120 to develop ISPR using adsorption capacity and desorption ratio as bases. The yield of PL in response to external PL was unaffected in shake-flask culture; however, the production of PL increased to 2.9 from 0.8 g l(-1) shake-flasks using ISPR. In a 5 l fermentor, 23.4 g PL l(-1) was achieved compared to 3.76 g PL l(-1), in the controls by attaching two bags of D152 resin to the probes and baffles of the fermentor.
采用树脂基原位产物去除（ISPR）技术提高链霉菌属 GIM8 生产 ε-聚赖氨酸（PL）的产量。以吸附容量和洗脱比为基础，从 Amberlite IRC-50、Amberlite IRC-76 和 Amberlite IR-120 中选择 D152 树脂进行 ISPR。在摇瓶培养中，对外源 PL 的响应 PL 产率不受影响；然而，采用 ISPR 可将 PL 的产量从摇瓶中的 0.8 g/L 提高到 2.9 g/L。在 5 L 发酵罐中，通过将两个 D152 树脂袋连接到发酵罐的探头和挡板上，与对照相比，实现了 23.4 g PL/L，而对照中仅为 3.76 g PL/L。
