Tong Siyu, Zhang Chen, Zhang Zhanyang, Zeng Huawei, Xin Bingyue, Zhao Mingtao, Zhao Deyin, Zeng Xin, Zhang Fei
Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, Huaibei Normal University, Huaibei 235000, China.
College of Life Sciences, Huaibei Normal University, Huaibei 235000, China.
Microorganisms. 2025 Aug 10;13(8):1868. doi: 10.3390/microorganisms13081868.
Epsilon-poly-l-lysine (ε-PL) is a potent antimicrobial agent, but strategies to enhance its biosynthesis remain limited due to insufficient understanding of its physiological regulation. This study explores the interaction between and heterogeneous microbial extracts, with a focus on actinomycete-derived signals. The extract induces the highest ε-PL production (3.4 g/L), exceeding the control by 2.6-fold and outperforming by 1.8-fold. Multi-omics analyses combined with morphological and biochemical profiling reveal that the induced state is characterized by intensified central carbon flux, enhanced lipid turnover, elevated respiratory activity, and cofactor regeneration, alongside suppression of competing secondary pathways. Morphological alterations, including denser mycelial aggregation and compact colony structures, accompany these metabolic shifts. Compared to , elicits more pronounced stress adaptation and metabolic reprogramming in . These findings suggest that interspecies interactions can activate intrinsic aggression resistance mechanisms, thereby driving ε-PL biosynthesis through a previously unrecognized physiological route.
ε-聚-L-赖氨酸（ε-PL）是一种强效抗菌剂，但由于对其生理调节的了解不足，增强其生物合成的策略仍然有限。本研究探讨了[未提及具体物质]与异源微生物提取物之间的相互作用，重点关注放线菌衍生的信号。[未提及具体物质]提取物诱导产生的ε-PL产量最高（3.4 g/L），比对照高出2.6倍，比[未提及具体物质]高出1.8倍。多组学分析结合形态学和生化分析表明，诱导状态的特征是中央碳通量增强、脂质周转加快、呼吸活性提高和辅因子再生，同时抑制竞争性次级途径。这些代谢变化伴随着形态学改变，包括更密集的菌丝聚集和紧凑的菌落结构。与[未提及具体物质]相比，[未提及具体物质]在[未提及具体物质]中引发更明显的应激适应和代谢重编程。这些发现表明，种间相互作用可以激活内在的抗侵袭机制，从而通过一条以前未被认识的生理途径驱动ε-PL的生物合成。
