Neurosurgery Center for Research, Training and Education, Loma Linda University, Loma Linda, California 92354, USA.
Magn Reson Med. 2012 Mar;67(3):711-7. doi: 10.1002/mrm.23045. Epub 2011 Jun 30.
Brain microbleeds (BMB) are associated with chronic and acute cerebrovascular disease. Because BMB present in the brain is a source of potentially cytotoxic iron proportional to the volume of extravasated blood, BMB iron content is a potentially valuable biomarker both to assess tissue risk and small cerebral vessel health. We recently reported methods to quantify focal iron sources using phase images that were tested in phantoms and BMB in postmortem tissue. In this study, we applied our methods to small hemorrhagic lesions induced in the in vivo rat brain using bacterial collagenase. As expected by theory, measurements of geometric features in phase images correlated with lesion iron content measured by graphite furnace atomic absorption spectrometry. Iron content estimation following BMB in an in vivo rodent model could shed light on the role and temporal evolution of iron-mediated tissue damage and efficacy of potential treatments in cerebrovascular diseases associated with BMB.
脑微出血（BMB）与慢性和急性脑血管病有关。由于脑内存在的 BMB 是潜在细胞毒性铁的来源，其与外渗血液的体积成正比，因此 BMB 铁含量是评估组织风险和小脑血管健康的一个有价值的潜在生物标志物。我们最近报道了使用相位图像定量聚焦铁源的方法，并在体模和死后组织中的 BMB 中进行了测试。在这项研究中，我们将我们的方法应用于使用细菌胶原酶在活体大鼠脑中诱导的小出血性病变。正如理论所预期的那样，相位图像中几何特征的测量与石墨炉原子吸收光谱法测量的病变铁含量相关。在活体啮齿动物模型中对 BMB 进行铁含量估计可以阐明铁介导的组织损伤的作用和时间演变，以及与 BMB 相关的脑血管病潜在治疗方法的疗效。
