Li Dandan, Zhào Hóngyi, Wei Wei, Liu Nan, Dr Huang Yonghua
Department of Neurology, Second Hospital of Shanxi Medical University; Department of Neurology, PLA Army General Hospital.
Department of Neurology, PLA Army General Hospital; Department of Neurology, NO 261 Hospital of PLA.
J Vis Exp. 2018 Oct 17(140):58423. doi: 10.3791/58423.
Cerebral microhemorrhages (CMHs) are common in aged patients and are correlated to various neuropsychiatric disorders. The etiology of CMHs is complex, and neuroinflammation is often observed as a co-occurrence. Here, we describe a sub-acute CMHs rat model induced by lipopolysaccharide (LPS) injection, as well as a method for detecting CMHs. Systemic LPS injection is relatively simple, economical, and cost-effective. One major advantage of LPS injection is its stability to induce inflammation. CMHs caused by LPS injection could be detected by gross observation, hematoxylin and eosin (HE) staining, Perl's Prussian staining, Evans blue (EB) double-labeling, and magnetic resonance imaging-susceptibility weighted imaging (MRI-SWI) technology. Finally, other methods of developing CMHs animal models, including their advantages and/or disadvantages, are also discussed in this report.
脑微出血（CMHs）在老年患者中很常见，并且与多种神经精神疾病相关。CMHs的病因复杂，常伴有神经炎症。在此，我们描述了一种通过注射脂多糖（LPS）诱导的亚急性CMHs大鼠模型以及一种检测CMHs的方法。全身注射LPS相对简单、经济且性价比高。注射LPS的一个主要优点是其诱导炎症的稳定性。通过大体观察、苏木精-伊红（HE）染色、Perl氏普鲁士蓝染色、伊文思蓝（EB）双标记以及磁共振成像-磁敏感加权成像（MRI-SWI）技术可检测到由LPS注射引起的CMHs。最后，本报告还讨论了其他建立CMHs动物模型的方法及其优缺点。
