Bar-Ilan University, The Mina & Everard Goodman Faculty of Life-Sciences, Ramat-Gan, 52900 Israel.
J Biomed Opt. 2011 Jun;16(6):067004. doi: 10.1117/1.3585674.
The involvement of mitochondria in pathological states, such as neurodegenerative diseases, sepsis, stroke, and cancer, are well documented. Monitoring of nicotinamide adenine dinucleotide (NADH) fluorescence in vivo as an intracellular oxygen indicator was established in 1950 to 1970 by Britton Chance and collaborators. We use a multiparametric monitoring system enabling assessment of tissue vitality. In order to use this technology in clinical practice, the commercial developed device, the CritiView (CRV), is tested in animal models as well as in patients.
The new CRV enables the optical monitoring of four different parameters, representing the energy balance of various tissues in vivo. Mitochondrial NADH is measured by surface fluorometry/reflectometry. In addition, tissue microcirculatory blood flow, tissue reflectance and oxygenation are measured as well. The device is tested both in vitro and in vivo in a small animal model and in preliminary clinical trials in patients undergoing vascular or open heart surgery. In patients, the monitoring is started immediately after the insertion of a three-way Foley catheter (urine collection) to the patient and is stopped when the patient is discharged from the operating room. The results show that monitoring the urethral wall vitality provides information in correlation to the surgical procedure performed.
线粒体在病理状态下的作用，如神经退行性疾病、败血症、中风和癌症等，已有大量文献记载。Britton Chance 及其合作者于 1950 年至 1970 年间建立了监测烟酰胺腺嘌呤二核苷酸（NADH）荧光作为细胞内氧指示剂的方法。我们使用多参数监测系统来评估组织活力。为了将这项技术应用于临床实践，我们对商业开发的设备 CritiView（CRV）进行了动物模型和患者的测试。
新型 CRV 能够光学监测四个不同的参数，代表体内各种组织的能量平衡。线粒体 NADH 通过表面荧光法/反射法进行测量。此外，还测量组织微循环血流、组织反射率和氧合作用。该设备在小动物模型中进行了体外和体内测试，并在接受血管或心脏直视手术的患者中进行了初步临床试验。在患者中，监测在插入三通 Foley 导管（尿液收集）到患者身上后立即开始，并在患者从手术室出院时停止。结果表明，监测尿道壁活力可提供与手术过程相关的信息。
