Bititalia, Milan, Italy.
J Altern Complement Med. 2011 Aug;17(8):701-4. doi: 10.1089/acm.2010.0439. Epub 2011 Jul 1.
Jaques Benveniste suggested that electronic transmission of 4-phorbol-12-b-myristate-13-acetate (PMA) activity, carried out using a simple amplifier configured to function as an oscillator when coupled to two electromagnetic coils, demonstrates the same chemical activity as the active molecule. The results obtained suggested that there are associated signals at the PMA molecules that can be transferred to target neutrophils by artificial physical means in a fashion that mimics the original molecules.
Retinoic acid was placed at room temperature on one coil attached to an oscillator (VEGA select 719), while LAN-5 neuroblastoma cells were placed on another coil and incubated under controlled condition. The oscillator was then turned on for 12 hours a day for 5 days, after which cells were counted and morphology studied by contrast microscopy.
The effect of the differentiating agent added to the cell culture by physical means generates a decrease in cell growth, metabolic activity, and the protrusion of a neuritelike structure typical of the differentiated cells.
These preliminary results suggest that retinoic acid molecules emit signals that can be transferred to LAN-5 neuroblastoma cells by artificial physical means in a manner that seems related to the chemical structure of the source molecules.
雅克·本维尼斯特（Jacques Benveniste）提出，使用配置为振荡器的简单放大器，当与两个电磁线圈耦合时，可传递 4-佛波醇-12-肉豆蔻酸-13-醋酸酯（PMA）的电子传递活动，从而证明与活性分子相同的化学活性。获得的结果表明，在 PMA 分子上存在相关信号，可以通过人工物理手段将其传递到靶中性粒细胞，从而模拟原始分子。
将维甲酸（retinoic acid）置于室温下的一个线圈上，该线圈与振荡器（VEGA select 719）相连，同时将 LAN-5 神经母细胞瘤置于另一个线圈上，并在受控条件下孵育。然后，振荡器每天开启 12 小时，持续 5 天，之后对细胞进行计数，并通过相差显微镜观察细胞形态。
通过物理手段添加到细胞培养物中的分化剂会导致细胞生长、代谢活性下降，以及出现类似于分化细胞的神经突样结构的突起。
这些初步结果表明，维甲酸分子发出的信号可以通过人工物理手段传递到 LAN-5 神经母细胞瘤，这种传递方式似乎与源分子的化学结构有关。
