由合成的IS50（Tn5）衍生物进行的分子内转座。
Intramolecular transposition by a synthetic IS50 (Tn5) derivative.
作者信息
Tomcsanyi T, Berg C M, Phadnis S H, Berg D E
机构信息
Department of Molecular Microbiology, Washington University Medical School, St. Louis, Missouri 63110-1093.
出版信息
J Bacteriol. 1990 Nov;172(11):6348-54. doi: 10.1128/jb.172.11.6348-6354.1990.
Abstract
We report the formation of deletions and inversions by intramolecular transposition of Tn5-derived mobile elements. The synthetic transposons used contained the IS50 O and I end segments and the transposase gene, a contraselectable gene encoding sucrose sensitivity (sacB), antibiotic resistance genes, and a plasmid replication origin. Both deletions and inversions were associated with loss of a 300-bp segment that is designated the vector because it is outside of the transposon. Deletions were severalfold more frequent than inversions, perhaps reflecting constraints on DNA twisting or abortive transposition. Restriction and DNA sequence analyses showed that both types of rearrangements extended from one transposon end to many different sites in target DNA. In the case of inversions, transposition generated 9-bp direct repeats of target sequences.
摘要
我们报道了通过Tn5衍生的移动元件的分子内转座形成缺失和倒位的情况。所使用的合成转座子包含IS50 O和I末端片段以及转座酶基因、编码蔗糖敏感性的反选择基因（sacB）、抗生素抗性基因和质粒复制起点。缺失和倒位都与一个300碱基对片段的丢失有关，该片段被称为载体，因为它位于转座子之外。缺失的发生频率比倒位高几倍，这可能反映了对DNA扭曲或流产转座的限制。限制性酶切和DNA序列分析表明，这两种类型的重排都从一个转座子末端延伸到靶DNA中的许多不同位点。在倒位的情况下，转座产生了靶序列的9碱基对直接重复序列。
相似文献
1
Intramolecular transposition by a synthetic IS50 (Tn5) derivative.由合成的IS50（Tn5）衍生物进行的分子内转座。
J Bacteriol. 1990 Nov;172(11):6348-54. doi: 10.1128/jb.172.11.6348-6354.1990.
2
IS50-mediated inverse transposition: specificity and precision.IS50介导的反向转座：特异性与精确性。
Gene. 1985;34(1):17-26. doi: 10.1016/0378-1119(85)90290-2.
3
Orientation of IS50 transposase gene and IS50 transposition.IS50转座酶基因的定位与IS50转座
J Bacteriol. 1989 Sep;171(9):5212-4. doi: 10.1128/jb.171.9.5212-5214.1989.
4
Tn5 insertion specificity is not influenced by IS50 end sequences in target DNA.Tn5插入特异性不受靶DNA中IS50末端序列的影响。
Mol Gen Genet. 1991 Aug;228(1-2):312-5. doi: 10.1007/BF00282482.
5
Inversions and deletions generated by a mini-gamma delta (Tn1000) transposon.由微型γδ（Tn1000）转座子产生的倒位和缺失。
J Bacteriol. 1994 Mar;176(5):1332-8. doi: 10.1128/jb.176.5.1332-1338.1994.
6
A comparison of intramolecular rearrangements promoted by transposons Tn5 and Tn10.转座子Tn5和Tn10促进的分子内重排的比较。
Proc Biol Sci. 1991 Apr 22;244(1309):1-9. doi: 10.1098/rspb.1991.0043.
7
Localization of action of the IS50-encoded transposase protein.由IS50编码的转座酶蛋白的作用定位
Genetics. 1986 Mar;112(3):421-7. doi: 10.1093/genetics/112.3.421.
8
Simple and efficient generation in vitro of nested deletions and inversions: Tn5 intramolecular transposition.体外简单高效地产生嵌套缺失和倒位：Tn5分子内转座。
Nucleic Acids Res. 1998 Apr 15;26(8):1927-33. doi: 10.1093/nar/26.8.1927.
9
Intramolecular transposition by Tn10.Tn10介导的分子内转座
Cell. 1989 Oct 20;59(2):373-83. doi: 10.1016/0092-8674(89)90298-5.
10
Control of transposon Tn5 transposition in Escherichia coli.大肠杆菌中转座子Tn5转座的控制
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7450-4. doi: 10.1073/pnas.79.23.7450.
引用本文的文献
1
MuA-mediated in vitro cloning of circular DNA: transpositional autointegration and the effect of MuB.MuA介导的环状DNA体外克隆：转座自整合及MuB的作用
Mol Genet Genomics. 2016 Jun;291(3):1181-91. doi: 10.1007/s00438-016-1175-2. Epub 2016 Feb 4.
2
Suicidal autointegration of sleeping beauty and piggyBac transposons in eukaryotic cells.睡美人转座子和猪尾巴转座子在真核细胞中的自杀性自我整合
PLoS Genet. 2014 Mar 13;10(3):e1004103. doi: 10.1371/journal.pgen.1004103. eCollection 2014 Mar.
3
A gene truncation strategy generating N- and C-terminal deletion variants of proteins for functional studies: mapping of the Sec1p binding domain in yeast Mso1p by a Mu in vitro transposition-based approach.一种用于功能研究的产生蛋白质N端和C端缺失变体的基因截短策略：通过基于Mu体外转座的方法定位酵母Mso1p中的Sec1p结合结构域。
Nucleic Acids Res. 2005 Jul 8;33(12):e104. doi: 10.1093/nar/gni102.
4
Cluster of type IV secretion genes in Helicobacter pylori's plasticity zone.幽门螺杆菌可塑性区域中IV型分泌基因簇
J Bacteriol. 2003 Jul;185(13):3764-72. doi: 10.1128/JB.185.13.3764-3772.2003.
5
Insertion of mini-IS605 and deletion of adjacent sequences in the nitroreductase (rdxA) gene cause metronidazole resistance in Helicobacter pylori NCTC11637.微小插入序列IS605插入及相邻序列缺失导致幽门螺杆菌NCTC11637的硝基还原酶（rdxA）基因产生甲硝唑耐药性。
Antimicrob Agents Chemother. 1999 Nov;43(11):2657-62. doi: 10.1128/AAC.43.11.2657.
6
Tn5/IS50 target recognition.Tn5/IS50靶标识别
Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10716-21. doi: 10.1073/pnas.95.18.10716.
7
Simple and efficient generation in vitro of nested deletions and inversions: Tn5 intramolecular transposition.体外简单高效地产生嵌套缺失和倒位：Tn5分子内转座。
Nucleic Acids Res. 1998 Apr 15;26(8):1927-33. doi: 10.1093/nar/26.8.1927.
8
IS10/Tn10 transposition efficiently accommodates diverse transposon end configurations.IS10/Tn10转座能有效地适应多种转座子末端构型。
EMBO J. 1996 Sep 16;15(18):5112-22.
9
pDUAL: a transposon-based cosmid cloning vector for generating nested deletions and DNA sequencing templates in vivo.pDUAL：一种基于转座子的黏粒克隆载体，用于在体内产生嵌套缺失和DNA测序模板。
Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7874-8. doi: 10.1073/pnas.90.16.7874.
10
Implications of Tn5-associated adjacent deletions.Tn5相关邻近缺失的影响。
J Bacteriol. 1993 Mar;175(5):1264-71. doi: 10.1128/jb.175.5.1264-1271.1993.
本文引用的文献
1
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.通过在大肠杆菌中进行DNA融合和克隆分析基因控制信号。
J Mol Biol. 1980 Apr;138(2):179-207. doi: 10.1016/0022-2836(80)90283-1.
2
Sequences essential for transposition at the termini of IS50.IS50末端转座所必需的序列。
Proc Natl Acad Sci U S A. 1983 Dec;80(23):7293-7. doi: 10.1073/pnas.80.23.7293.
3
Specificity of transposon Tn5 insertion.转座子Tn5插入的特异性
Genetics. 1983 Dec;105(4):813-28. doi: 10.1093/genetics/105.4.813.
4
DNA sequences at the ends of transposon Tn5 required for transposition.转座所需的转座子Tn5末端的DNA序列。
Nature. 1983;304(5923):280-2. doi: 10.1038/304280a0.
5
Structural requirement for IS50-mediated gene transposition.IS50介导的基因转座的结构要求。
Proc Natl Acad Sci U S A. 1983 Feb;80(3):792-6. doi: 10.1073/pnas.80.3.792.
6
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.pUC质粒，一种源自M13mp7的用于插入诱变和使用合成通用引物进行测序的系统。
Gene. 1982 Oct;19(3):259-68. doi: 10.1016/0378-1119(82)90015-4.
7
Oligonucleotide-directed double-strand break repair in plasmids of Escherichia coli: a method for site-specific mutagenesis.大肠杆菌质粒中寡核苷酸定向双链断裂修复：一种位点特异性诱变方法。
Proc Natl Acad Sci U S A. 1986 Oct;83(19):7177-81. doi: 10.1073/pnas.83.19.7177.
8
A correction in the nucleotide sequence of the Tn903 kanamycin resistance determinant in pUC4K.pUC4K中Tn903卡那霉素抗性决定簇核苷酸序列的校正。
Nucleic Acids Res. 1988 Jan 11;16(1):358. doi: 10.1093/nar/16.1.358.
9
An nptI-sacB-sacR cartridge for constructing directed, unmarked mutations in gram-negative bacteria by marker exchange-eviction mutagenesis.一种用于通过标记交换-消除诱变在革兰氏阴性细菌中构建定向、无标记突变的nptI-sacB-sacR盒式载体。
Gene. 1987;57(2-3):239-46. doi: 10.1016/0378-1119(87)90127-2.
10
Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria.革兰氏阴性菌中插入序列元件捕获的阳性选择程序。
J Bacteriol. 1985 Nov;164(2):918-21. doi: 10.1128/jb.164.2.918-921.1985.
Zotero 插件