IS50末端转座所必需的序列。
Sequences essential for transposition at the termini of IS50.
作者信息
Sasakawa C, Carle G F, Berg D E
出版信息
Proc Natl Acad Sci U S A. 1983 Dec;80(23):7293-7. doi: 10.1073/pnas.80.23.7293.
The DNA sequences found repeated in opposite orientation at the ends of insertion (IS) elements are thought to contain sites at which transposase proteins act during transposition. Many elements have repeats of at least 15 base pairs (bp). Those of IS50 are quite short, however: just 8 of the first 9 bp. Functional tests had indicated that one end of IS50 is more effective in transposition than the other end and suggested that at least one of the recognition sites of IS50 extends beyond the common 8/9 bp. To determine the lengths of recognition sites of IS50 we mutagenized IS50 in vitro and tested the transposition proficiency of the resulting mutants. Our results show that the recognition sites at each end of IS50 are about 19 bp long. These findings suggest models for the evolution of IS elements from simpler immobile gene complexes.
在插入（IS）元件末端以相反方向重复出现的DNA序列被认为包含转座酶蛋白在转座过程中作用的位点。许多元件具有至少15个碱基对（bp）的重复序列。然而，IS50的重复序列相当短：前9个bp中只有8个。功能测试表明，IS50的一端在转座中比另一端更有效，并表明IS50的识别位点中至少有一个超出了常见的8/9 bp。为了确定IS50识别位点的长度，我们在体外对IS50进行诱变，并测试所得突变体的转座能力。我们的结果表明，IS50两端的识别位点长度约为19 bp。这些发现为IS元件从更简单的固定基因复合体进化而来提供了模型。
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