用于鉴定和区分鲁氏耶尔森氏菌生物型 2 菌株的快速基因分型检测方法。
Rapid genotyping assays for the identification and differentiation of Yersinia ruckeri biotype 2 strains.
机构信息
National Center for Cool and Cold Water Aquaculture, Agricultural Research Service, US Department of Agriculture, Kearneysville, WV 25430, USA.
出版信息
Lett Appl Microbiol. 2011 Sep;53(3):383-5. doi: 10.1111/j.1472-765X.2011.03114.x. Epub 2011 Jul 18.
AIMS
To establish PCR-based assays for the rapid identification and differentiation of each of four known biotype 2 (BT2) phenotype-causing alleles in Yersinia ruckeri strains currently circulating in Europe and the United States.
METHODS AND RESULTS
Novel assays were developed relying on detection of mutant allele-specific changes in restriction enzyme cleavage sites within targeted PCR products. The developed assays were validated against isolates previously genotyped by DNA sequencing.
CONCLUSIONS
The described methods were specific, rapid and simple to perform and interpret.
SIGNIFICANCE AND IMPACT OF THE STUDY
The developed genotyping assays provide a valuable tool for identification and differentiation of specific BT2 strains of Y. ruckeri. These assays will be critical for the design and validation of new vaccines or other measures meant to control BT2 strains.
目的
建立基于 PCR 的方法，快速鉴定和区分当前在欧洲和美国流行的四种已知生物型 2（BT2）表型致因等位基因。
方法和结果
本研究依赖于检测目标 PCR 产物中突变等位基因特异性的限制酶切割位点变化，开发了新的检测方法。通过 DNA 测序对已鉴定的分离株进行了验证。
结论
描述的方法具有特异性、快速且易于操作和解释。
研究的意义和影响
所开发的基因分型方法为鉴定和区分特定的 Y. ruckeri BT2 菌株提供了有价值的工具。这些方法对于设计和验证旨在控制 BT2 菌株的新型疫苗或其他措施至关重要。
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