土耳其虹鳟（Oncorhynchus mykiss，Walbaum，1792）分离的鲁氏耶尔森菌的表型和分子特征

Phenotypic and molecular characterization of Yersinia ruckeri isolates from rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) in Turkey.

作者信息

Onuk Ertan Emek, Ciftci Alper, Findik Arzu, Ciftci Gülay, Altun Soner, Balta Fikri, Ozer Selmin, Coban Ahmet Yilmaz

机构信息

Department of Diseases and Clinical Sciences, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun,Turkey.

出版信息

Berl Munch Tierarztl Wochenschr. 2011 Jul-Aug;124(7-8):320-8.

PMID:21848040

Abstract

The aim of the study was the phenotypic and molecular characterization of Yersinia (Y) ruckeri strains, the causative agent of Enteric Redmouth Disease (ERM), by antibiotyping, random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) and sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns of whole cell proteins. For this aim, a total of 97 Y ruckeri isolates were analyzed. The isolates were distinguished into ten antibiotypes and six phenotypes according to their resistance properties and whole cell protein profiles, respectively. Also, a glycoprotein band of approximately 25.5 kDa was observed in all Y ruckeri strains tested. In all strains, six different RAPD types were observed. In conclusion, Y ruckeri strains isolated from rainbow trout of fish farms in Turkey showed variation according to their phenotypic and genotypic characteristics, and the use of these three typing techniques in double and triple combinations could be more useful for discriminating the strains.

摘要

本研究的目的是通过抗菌谱分型、随机扩增多态性DNA聚合酶链反应（RAPD-PCR）以及全细胞蛋白的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）图谱，对肠红嘴病（ERM）病原体鲁氏耶尔森菌（Yersinia, Y）菌株进行表型和分子特征分析。为此，共分析了97株鲁氏耶尔森菌分离株。根据其耐药特性和全细胞蛋白谱，分离株分别被分为十种抗菌型和六种表型。此外，在所有测试的鲁氏耶尔森菌菌株中均观察到一条约25.5 kDa的糖蛋白条带。在所有菌株中，观察到六种不同的RAPD类型。总之，从土耳其养鱼场虹鳟鱼中分离出的鲁氏耶尔森菌菌株根据其表型和基因型特征表现出差异，将这三种分型技术双重或三重组合使用可能更有助于区分菌株。