Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing Key Laboratory, Beijing 100875, PR China.
Immunol Lett. 2011 Oct 30;140(1-2):52-8. doi: 10.1016/j.imlet.2011.06.004. Epub 2011 Jun 21.
Protective immunity involves a dynamic balance between humoral and cellular immune responses. In the present work we demonstrated that recombinant human calcineurin subunit B (rhCnB) stimulated the expression of the surface molecules CD83, CD80, CD86, CD40, and HLA-DR. It also promoted secretion of inflammatory cytokines IL-6, TNF-α, and IL-1β by human PBMC-derived dendritic cells. In in vivo experiments, splenocytes from BALB/c mice immunized with pneumolysin plus rhCnB contained a higher percentage of CD3(+)CD4(+) T lymphocytes, produced more antigen-specific splenocyte proliferation activity, and had higher anti-pneumolysin immunoglobulin G (IgG) titers. Transcript levels of cytokines such as IL-4, IL-10, and IFN-γ in the splenocytes were also upregulated when in vitro stimulated with pneumolysin. Thus, rhCnB promoted a mixed Th1/Th2 type immune response when given together with the specific antigen PN. RhCnB could have potential as a prophylactic vaccine adjuvant.
保护性免疫涉及体液免疫和细胞免疫应答之间的动态平衡。在本工作中，我们证明重组人钙调神经磷酸酶亚基 B（rhCnB）可刺激人外周血单核细胞来源的树突状细胞表面分子 CD83、CD80、CD86、CD40 和 HLA-DR 的表达。它还可促进炎性细胞因子 IL-6、TNF-α 和 IL-1β 的分泌。在体内实验中，肺炎球菌溶血素加 rhCnB 免疫的 BALB/c 小鼠脾细胞含有更高比例的 CD3(+)CD4(+)T 淋巴细胞，产生更多的抗原特异性脾细胞增殖活性，并且具有更高的抗肺炎球菌溶血素免疫球蛋白 G（IgG）滴度。当用肺炎球菌溶血素体外刺激时，脾细胞中的细胞因子如 IL-4、IL-10 和 IFN-γ 的转录水平也上调。因此，rhCnB 与特异性抗原 PN 一起使用时可促进混合 Th1/Th2 型免疫应答。rhCnB 可能具有作为预防性疫苗佐剂的潜力。
